Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I2 R1
|
67 |
36.0 |
1614110 |
95.1% |
1535018 |
114.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,553,049 |
A→G |
D50G (GAC→GGC) |
uxuB → |
D‑mannonate oxidoreductase, NAD‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,553,049 | 0 | A | G | 100.0%
| 90.8
/ NA
| 25 | D50G (GAC→GGC) | uxuB | D‑mannonate oxidoreductase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/11); total (14/11) |
TTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGA > NC_000913/4552914‑4553174
|
ttgACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAg > 1:769222/1‑139 (MQ=255)
cAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTgg > 1:129037/1‑139 (MQ=255)
ccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAAcc < 2:88395/139‑1 (MQ=255)
ccgTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAAcc < 1:280698/139‑1 (MQ=255)
aTCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAgg < 2:119048/139‑1 (MQ=255)
aTTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCaa > 2:783270/1‑139 (MQ=255)
aTTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCaa < 2:139823/139‑1 (MQ=255)
ttCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAAc > 1:705056/1‑139 (MQ=255)
tgCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTAAACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCt > 1:68710/1‑139 (MQ=255)
tgCATCTCGGTTGCGGGGCATTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCt > 2:337970/1‑139 (MQ=255)
tGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGc < 2:68710/139‑1 (MQ=255)
cgcTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa < 1:371078/113‑1 (MQ=255)
cgcTGTATACCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa > 2:371078/1‑113 (MQ=255)
ataCCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTg < 2:254285/80‑1 (MQ=255)
ataCCCATCATCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTg > 1:254285/1‑80 (MQ=255)
catcatCTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCa < 1:337970/139‑1 (MQ=255)
tcatcTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCAcc > 2:260887/1‑139 (MQ=255)
atcTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa > 1:726864/1‑98 (MQ=255)
atcTGCTGGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa < 2:726864/98‑1 (MQ=255)
ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGgc < 2:584645/120‑1 (MQ=255)
ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGgc > 1:584645/1‑120 (MQ=255)
ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGa > 2:360662/1‑139 (MQ=255)
ctgGAAAGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGa > 1:18048/1‑139 (MQ=255)
aaGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa < 2:181091/88‑1 (MQ=255)
aaGCACCGGCAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCaa > 1:181091/1‑88 (MQ=255)
|
TTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCATCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGGGCATCTGCGAAGTTAACCTGATGCCAGGCAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACCGTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGA > NC_000913/4552914‑4553174
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
