Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	83	73.5	3575638	92.3%	3300313	107.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,596	C→T	A29T (GCC→ACC)	uxaB ←	altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,596	0	C	T	100.0%	250.5 / NA	72	A29T (GCC→ACC)	uxaB	altronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (35/37); total (35/37)

GATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGA  >  NC_000913/1610462‑1610728

gATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGgtgcgc                                                                                                                                  <  2:1322038/139‑1 (MQ=255)

      ggtggtGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAg                                                                                                                            <  2:690255/139‑1 (MQ=255)

                aGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTAc                                                                                                                        >  1:1675820/1‑133 (MQ=255)

                aGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTAc                                                                                                                        <  2:1675820/133‑1 (MQ=255)

                 gACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCAcc                                                                                                                 >  1:732912/1‑139 (MQ=255)

                  aCCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCa                                                                                                                <  1:486463/139‑1 (MQ=255)

                  aCCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCa                                                                                                                >  2:450890/1‑139 (MQ=255)

                    cATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCaaa                                                                                                              <  2:481833/139‑1 (MQ=255)

                      tCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACt                                                                                                            >  2:138262/1‑139 (MQ=255)

                       ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTg                                                                                                           >  2:342752/1‑139 (MQ=255)

                       ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTg                                                                                                           >  1:1259345/1‑139 (MQ=255)

                            gTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAg                                                                                                                            <  2:1026744/117‑1 (MQ=255)

                            gTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAg                                                                                                                            >  1:1026744/1‑117 (MQ=255)

                            gTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGa                                                                                                      >  1:741920/1‑139 (MQ=255)

                             tGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt                                                                                                     <  1:138262/139‑1 (MQ=255)

                                            ggAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCa                                                                                                                   >  2:259415/1‑110 (MQ=255)

                                            ggAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCa                                                                                                                   <  1:259415/110‑1 (MQ=255)

                                            ggAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc                                                                                                   >  2:307775/1‑126 (MQ=255)

                                            ggAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc                                                                                                   <  1:307775/126‑1 (MQ=255)

                                            ggAATGAAGATTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACtg                                                                                      >  2:1197724/1‑139 (MQ=255)

                                               aTGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGc                                                                                   <  1:429615/139‑1 (MQ=255)

                                                tGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCa                                                                                  <  2:1142451/139‑1 (MQ=255)

                                                 gAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCAc                                                                                 <  1:1197724/139‑1 (MQ=255)

                                                      ttCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc                                                                                                   >  2:954385/1‑116 (MQ=255)

                                                      ttCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc                                                                                                   <  1:954385/116‑1 (MQ=255)

                                                       tCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGTTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaa                                                                           >  2:751291/1‑139 (MQ=255)

                                                       tCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCa                                                                                                                                 <  1:507228/85‑1 (MQ=255)

                                                       tCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCa                                                                                                                                 >  2:507228/1‑85 (MQ=255)

                                                              gACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATcgcg                                                                    <  1:564615/139‑1 (MQ=255)

                                                              gACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATcgcg                                                                    <  2:741920/139‑1 (MQ=255)

                                                                     aacGACCACGCCAGAATTCAGATCGGTGTGTTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGAttt                                                             <  1:751291/139‑1 (MQ=255)

                                                                         aCCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAgtg                                                         >  2:1300648/1‑139 (MQ=255)

                                                                         aCCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAgtg                                                         >  2:788410/1‑139 (MQ=255)

                                                                             cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAAt                                                                        <  1:29527/120‑1 (MQ=255)

                                                                             cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAAt                                                                        >  2:29527/1‑120 (MQ=255)

                                                                             cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGtttt                                                     >  2:598853/1‑139 (MQ=255)

                                                                             cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGtttt                                                     >  2:1297849/1‑139 (MQ=255)

                                                                             cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAAGCGCGACGATTTAGTGtttt                                                     >  2:805153/1‑139 (MQ=255)

                                                                                      tCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATggg                                            <  1:1297849/139‑1 (MQ=255)

                                                                                        aGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt                                                                                                     >  1:846473/1‑80 (MQ=255)

                                                                                        aGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt                                                                                                     <  2:846473/80‑1 (MQ=255)

                                                                                        aGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGtt                                          <  1:1300648/139‑1 (MQ=255)

                                                                                           tCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTccc                                       <  1:598853/139‑1 (MQ=255)

                                                                                             ggTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCAGCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCtt                                     <  1:1264419/139‑1 (MQ=255)

                                                                                             ggTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGtt                                          <  2:473596/134‑1 (MQ=255)

                                                                                             ggTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGtt                                          >  1:473596/1‑134 (MQ=255)

                                                                                               tgtgCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCt                                   >  2:1720035/1‑139 (MQ=255)

                                                                                                   cTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGAtt                               <  2:732912/139‑1 (MQ=255)

                                                                                                   cTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGAtt                               <  1:788410/139‑1 (MQ=255)

                                                                                                       ttCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCACTTCTGATTAGTc                           >  1:983550/1‑139 (MQ=255)

                                                                                                        tCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAg                                                           >  1:275521/1‑106 (MQ=255)

                                                                                                        tCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAg                                                           <  2:275521/106‑1 (MQ=255)

                                                                                                            gagaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAATTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAg                                                           <  1:1304066/102‑1 (MQ=255)

                                                                                                            gagaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAg                                                           >  2:1571519/1‑102 (MQ=255)

                                                                                                            gagaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAg                                                           >  2:1304066/1‑102 (MQ=255)

                                                                                                            gagaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAg                                                           <  1:1571519/102‑1 (MQ=255)

                                                                                                             agaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt                                                                                                     <  2:1729010/59‑1 (MQ=255)

                                                                                                             agaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt                                                                                                     >  1:1729010/1‑59 (MQ=255)

                                                                                                               aTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCaaa                                                                                                              >  2:170668/1‑48 (MQ=255)

                                                                                                               aTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCaaa                                                                                                              <  1:170668/48‑1 (MQ=255)

                                                                                                               aTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATa                                                                                         >  1:1535846/1‑69 (MQ=255)

                                                                                                               aTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATa                                                                                         <  2:1535846/69‑1 (MQ=255)

                                                                                                               aTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAgt                                                          <  1:1637604/100‑1 (MQ=255)

                                                                                                               aTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAgt                                                          >  2:1637604/1‑100 (MQ=255)

                                                                                                                tCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc                                                                                                   <  2:1384393/58‑1 (MQ=255)

                                                                                                                tCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc                                                                                                   >  1:1384393/1‑58 (MQ=255)

                                                                                                                    tttGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt                                                                                                     <  1:225076/52‑1 (MQ=255)

                                                                                                                    tttGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt                                                                                                     >  2:225076/1‑52 (MQ=255)

                                                                                                                          aGTCAACAAAGGTGCGCAGGAAGTTACC‑TCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTgg       >  1:680708/1‑139 (MQ=255)

                                                                                                                           gTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTgg       <  1:539724/139‑1 (MQ=255)

                                                                                                                              aacaaAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTAc    >  1:1218176/1‑139 (MQ=255)

                                                                                                                                caaAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGa  <  2:1218176/139‑1 (MQ=255)

                                                                                                                                caaAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGa  <  2:834731/139‑1 (MQ=255)

GATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGA  >  NC_000913/1610462‑1610728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A