Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
20.9 |
912876 |
97.7% |
891879 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,613,082 |
T→C |
D57G (GAC→GGC) |
glsB ← |
glutaminase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,613,082 | 0 | T | C | 100.0%
| 44.3
/ NA
| 14 | D57G (GAC→GGC) | glsB | glutaminase 2 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (4/10); total (4/10) |
AGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGTCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCC > NC_000913/1612970‑1613183
|
aGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCa < 1:96252/139‑1 (MQ=255)
gACGGATCTTTGCCGACGCGTTGCCAGATTTCCTTTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCaa < 2:201454/139‑1 (MQ=255)
aCGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAAc < 2:73301/139‑1 (MQ=255)
ggATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTcc < 1:427446/131‑1 (MQ=255)
ggATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTcc > 2:427446/1‑131 (MQ=255)
aCGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTGGGAAATAGACTGAATGGGAAAACGGTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCaa > 2:21002/1‑139 (MQ=255)
ggCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt < 1:329194/83‑1 (MQ=255)
ggCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt > 2:329194/1‑83 (MQ=255)
gCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt < 1:344439/82‑1 (MQ=255)
gCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt > 2:344439/1‑82 (MQ=255)
gagaCTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATcc < 2:251558/139‑1 (MQ=255)
agaCTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCcg < 1:21002/139‑1 (MQ=255)
agaCTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCcg < 2:436157/139‑1 (MQ=255)
tttGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTTCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTAccc < 1:444125/139‑1 (MQ=255)
|
AGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGTCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCC > NC_000913/1612970‑1613183
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
