Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	83	73.5	3575638	92.3%	3300313	107.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,102	G→A	A45V (GCA→GTA)	yoaA ←	putative ATP‑dependent helicase, DinG family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,102	0	G	A	100.0%	99.1 / NA	30	A45V (GCA→GTA)	yoaA	putative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/16); total (14/16)

TTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAA  >  NC_000913/1892971‑1893224

ttGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccac                                                                                                                     >  1:680889/1‑139 (MQ=255)

ttGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccac                                                                                                                     >  1:1485167/1‑139 (MQ=255)

       tGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGc                                                                                                              >  1:326131/1‑139 (MQ=255)

          tGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGc                                                                                                           <  2:680889/139‑1 (MQ=255)

          tGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGc                                                                                                           <  1:1760130/139‑1 (MQ=255)

           ggCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCt                                                                                                          >  2:57080/1‑139 (MQ=255)

           ggCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCt                                                                                                          >  2:1739490/1‑139 (MQ=255)

                       cTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTa                                                                                              <  1:1383963/139‑1 (MQ=255)

                        tGTAGGGCTGATCCTGCTACGCTTTTGAGCCGGTCGAGATAAGGACTTTCTTTTTCGACCGCAGCGCAGGAGCCAGGTAAGCGTAGTTTTTGCCCGTACCGGTTCCTACTTCCACCACCTGCGGCTGGCCTTTTTCTAt                                                                                             <  1:824502/139‑1 (MQ=255)

                            gagCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGcc                                                                                         <  1:1698172/139‑1 (MQ=255)

                                  aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCt                                                                                        >  1:1518655/1‑134 (MQ=255)

                                  aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCt                                                                                        <  2:1518655/134‑1 (MQ=255)

                                  aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg                                                                                   <  1:1739490/139‑1 (MQ=255)

                                  aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg                                                                                   >  2:1417161/1‑139 (MQ=255)

                                  aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg                                                                                   <  2:1043498/139‑1 (MQ=255)

                                                      cGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCg                                                               <  1:1417161/139‑1 (MQ=255)

                                                                  tGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTcg                                                   <  2:1145736/139‑1 (MQ=255)

                                                                  tGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTcg                                                   >  1:1145736/1‑139 (MQ=255)

                                                                   gACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTcgc                                                  <  1:1012035/139‑1 (MQ=255)

                                                                        tctttTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCtt                                             >  2:1505615/1‑139 (MQ=255)

                                                                         ctttTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTATATCGCATGGGTGACGGCTACCACCATCTGTCGCTGTGGTTCTCGCGGCTTa                                            <  1:1032979/139‑1 (MQ=255)

                                                                                                  aGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCtgt                                                           >  2:1062263/1‑99 (MQ=255)

                                                                                                  aGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCtgt                                                           <  1:1062263/99‑1 (MQ=255)

                                                                                                  aGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCt                   >  1:1774349/1‑139 (MQ=255)

                                                                                                      aaGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGAcc               <  2:326131/139‑1 (MQ=255)

                                                                                                             ggTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGt        >  2:1510546/1‑139 (MQ=255)

                                                                                                                   gCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCaaaa  <  2:1774349/139‑1 (MQ=255)

                                                                                                                   gCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCaaaa  >  1:1345532/1‑139 (MQ=255)

                                                                                                                              ttCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCGTCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTa                                  <  1:330378/96‑1 (MQ=255)

                                                                                                                              ttCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTa                                  >  2:330378/1‑96 (MQ=255)

TTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAA  >  NC_000913/1892971‑1893224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A