Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	83	73.5	3575638	92.3%	3300313	107.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,867,926	(C)_6→7	coding (828/1140 nt)	nlpD ←	activator of AmiC murein hydrolase activity, lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,867,920	1	.	C	96.8%	228.7 / ‑0.7	63	coding (834/1140 nt)	nlpD	activator of AmiC murein hydrolase activity, lipoprotein
Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (31/30); total (33/30)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑C‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCG  >  NC_000913/2867783‑2868044

attaGGTAATTCTTATTTTGTTTGAATATTATCCGAATAACGTAGGCCCGCCACCCCGTAACAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               <  2:581724/136‑1 (MQ=255)

     gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTGGCGATAATTACCTGTCCTTGGCTGCCTACAATAACAATCCCCTTGTTA‑c‑ccc‑cc                                                                                                                        >  1:581724/1‑139 (MQ=255)

       aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               >  1:1274771/1‑132 (MQ=255)

       aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               <  2:1274771/132‑1 (MQ=255)

           atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCCATCCCCTTGTTGCC‑CCC‑CCTCAGa                                                                                                                   >  2:907016/1‑139 (MQ=255)

           atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGa                                                                                                                   >  1:1507128/1‑139 (MQ=255)

             catTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAg                                                                                                                 >  2:1359663/1‑139 (MQ=255)

              atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               >  1:1717002/1‑125 (MQ=255)

              atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               <  2:1717002/125‑1 (MQ=255)

               ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAgcg                                                                                                               >  2:464208/1‑139 (MQ=255)

                  tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCa                                                                                                            <  1:237881/139‑1 (MQ=255)

                     ttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAg                                                                                                         <  2:1400436/139‑1 (MQ=255)

                      tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            <  1:485022/119‑1 (MQ=255)

                      tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            >  2:485022/1‑119 (MQ=255)

                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGt                                                                                                       >  1:1138287/1‑139 (MQ=255)

                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGt                                                                                                       >  1:189757/1‑139 (MQ=255)

                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               >  2:1089217/1‑116 (MQ=255)

                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               <  1:1089217/116‑1 (MQ=255)

                            tAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  2:1626331/1‑139 (MQ=255)

                              aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  <  1:129471/137‑1 (MQ=255)

                              aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  2:129471/1‑137 (MQ=255)

                                      aCCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGcc                                                                                        <  2:1138287/139‑1 (MQ=255)

                                        cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      <  1:1195975/139‑1 (MQ=255)

                                        cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      >  1:1288700/1‑139 (MQ=255)

                                          tggCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            <  1:1071760/97‑1 (MQ=255)

                                          tAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            >  2:1071760/1‑99 (MQ=255)

                                             ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAg                                                                                                         >  1:691937/1‑115 (MQ=255)

                                             ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAg                                                                                                         <  2:691937/115‑1 (MQ=255)

                                             ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTc                                                                                 >  2:1217741/1‑139 (MQ=255)

                                                cgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGc                                                                              <  1:907016/139‑1 (MQ=255)

                                                 gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      <  2:382077/130‑1 (MQ=255)

                                                 gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTCTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑C‑CCCACCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      >  1:382077/1‑130 (MQ=255)

                                                 gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAT‑ATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCa                                                                            >  2:846226/1‑139 (MQ=255)

                                                  cAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               >  1:347720/1‑89 (MQ=255)

                                                  cAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               <  2:347720/89‑1 (MQ=255)

                                                     cgcgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgcg                                                                         <  2:239495/139‑1 (MQ=255)

                                                             cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgcgc                                                                        >  2:608375/1‑132 (MQ=255)

                                                             cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgcgc                                                                        <  1:608375/132‑1 (MQ=255)

                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGaa                                                                                                                  <  2:815830/83‑1 (MQ=255)

                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGaa                                                                                                                  >  1:815830/1‑83 (MQ=255)

                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGt                                                                                                       <  1:1276868/94‑1 (MQ=255)

                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGt                                                                                                       >  2:1276868/1‑94 (MQ=255)

                                                                     acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACt                                                                                             <  2:55643/103‑1 (MQ=255)

                                                                     acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACt                                                                                             >  1:55643/1‑103 (MQ=255)

                                                                         cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTAc                                                     >  1:1445931/1‑139 (MQ=255)

                                                                         cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTAc                                                     >  1:196358/1‑139 (MQ=255)

                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                    >  2:370517/1‑139 (MQ=255)

                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                    <  2:196358/139‑1 (MQ=255)

                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                    <  1:1626331/139‑1 (MQ=255)

                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            <  1:1675851/67‑1 (MQ=255)

                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            >  2:1675851/1‑67 (MQ=255)

                                                                             gCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGt                                                 <  2:1507128/139‑1 (MQ=255)

                                                                             gCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            >  2:1751380/1‑64 (MQ=255)

                                                                             gCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c‑cc                                                                                                                            <  1:1751380/64‑1 (MQ=255)

                                                                              ccATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGtt                                                >  2:414871/1‑139 (MQ=255)

                                                                                 tCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGt                                                                   >  2:1536851/1‑117 (MQ=255)

                                                                                 tCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGt                                                                   <  1:1536851/117‑1 (MQ=255)

                                                                                          gcgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               <  2:608136/49‑1 (MQ=255)

                                                                                          gcgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                                               >  1:608136/1‑49 (MQ=255)

                                                                                            gATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  <  1:831006/75‑1 (MQ=255)

                                                                                            gATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  2:831006/1‑75 (MQ=255)

                                                                                                  tGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCgg                            <  1:137398/139‑1 (MQ=255)

                                                                                                      tGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTActgct                                     <  1:713744/126‑1 (MQ=255)

                                                                                                      tGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTActgct                                     >  2:713744/1‑126 (MQ=255)

                                                                                                        tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCt                          >  1:752646/1‑135 (MQ=255)

                                                                                                        tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCt                          <  2:752646/135‑1 (MQ=255)

                                                                                                        tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CGCCC‑CCTCAGCAGCGCCAAAGGTTTCTATCATCTTGCCCTCAGGCGGCCAGCGCCAGGTGGAGATAGGCGTACGGGTTGCTGTGCTGCTGACAGTCGGCTCGGt                      >  1:1727903/1‑139 (MQ=255)

                                                                                                               ctgcctgcGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgctt               <  2:189757/139‑1 (MQ=255)

                                                                                                                  cctgcGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                                                           >  1:619354/1‑92 (MQ=255)

                                                                                                                  cctgcGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                                                           <  2:619354/92‑1 (MQ=255)

                                                                                                                  cctgcGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTa                                          <  2:35817/109‑1 (MQ=255)

                                                                                                                  cctgcGATATCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTa                                          >  1:35817/1‑109 (MQ=255)

                                                                                                                         tatCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCgg                       >  2:198199/1‑121 (MQ=255)

                                                                                                                         tatCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCgg                       <  1:198199/121‑1 (MQ=255)

                                                                                                                         tatCAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTa     <  2:1445931/139‑1 (MQ=255)

                                                                                                                            cAATCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCg  >  1:356828/1‑139 (MQ=255)

                                                                                                                               tCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGAt                                                                                                 <  1:685295/41‑1 (MQ=255)

                                                                                                                               tCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGAt                                                                                                 >  2:685295/1‑41 (MQ=255)

                                                                                                                               tCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTgg       >  1:552810/1‑131 (MQ=255)

                                                                                                                               tCCCCTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTgg       <  2:552810/131‑1 (MQ=255)

                                                                                                                                   cTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  1:1562773/1‑36 (MQ=255)

                                                                                                                                   cTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  <  2:1562773/36‑1 (MQ=255)

                                                                                                                                   cTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgct                <  2:649926/118‑1 (MQ=255)

                                                                                                                                   cTTGTTGCC‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgct                >  1:649926/1‑118 (MQ=255)

CTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑C‑CCC‑CCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCG  >  NC_000913/2867783‑2868044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A