Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
83 |
73.5 |
3575638 |
92.3% |
3300313 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,229,765 |
A→G |
A126A (GCT→GCC) |
pepE ← |
peptidase E, alpha‑aspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,229,765 | 0 | A | G | 98.2%
| 207.5
/ NA
| 57 | A126A (GCT→GCC) | pepE | peptidase E, alpha‑aspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (33/23); minor base T (0/1); total (33/24) |
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
ACGGGTTTCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGA > NC_000913/4229630‑4229898
|
aCGGGTTTCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGggcaggca < 2:1035855/139‑1 (MQ=255)
tCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAg > 2:372456/1‑139 (MQ=255)
tCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAg > 2:420016/1‑139 (MQ=255)
ccTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 2:1468251/139‑1 (MQ=255)
ccTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 1:818847/139‑1 (MQ=255)
ccTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 1:353956/139‑1 (MQ=255)
ttCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 2:732305/139‑1 (MQ=255)
gcgcGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAAtata > 1:1669672/1‑139 (MQ=255)
gcgTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCAACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACa < 2:1199530/139‑1 (MQ=255)
gTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACaga > 1:322544/1‑139 (MQ=255)
ggTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 1:491332/122‑1 (MQ=255)
ggTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc > 2:491332/1‑122 (MQ=255)
gTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAgcgc < 2:322544/139‑1 (MQ=255)
gTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAgcgc < 2:1337127/139‑1 (MQ=255)
tGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAAc < 2:1278640/131‑1 (MQ=255)
tGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGAGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAAc > 1:1278640/1‑131 (MQ=255)
aGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTaa < 2:1256552/139‑1 (MQ=255)
gCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAAt > 1:356111/1‑139 (MQ=255)
ggAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAgc < 2:714472/85‑1 (MQ=255)
ggAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAgc > 1:714472/1‑85 (MQ=255)
gcATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGCACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCcgc > 2:559638/1‑139 (MQ=255)
aTCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCgcgc < 1:420016/139‑1 (MQ=255)
tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGTCAGGCAAGGTTAGCGCCTGCGCTCCAg > 2:419592/1‑81 (MQ=255)
tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 1:419592/81‑1 (MQ=255)
tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCt > 2:365188/1‑139 (MQ=255)
tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCt > 1:563534/1‑139 (MQ=255)
tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCt > 1:378320/1‑139 (MQ=255)
ccTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGc < 2:378320/139‑1 (MQ=255)
cTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCa < 1:372456/139‑1 (MQ=255)
cGGATCGACAATCGGCTTATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTaa < 1:830262/107‑1 (MQ=255)
cGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTaa > 2:830262/1‑107 (MQ=255)
cGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAg > 1:691200/1‑139 (MQ=255)
gACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGc > 2:978404/1‑139 (MQ=255)
gACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGc > 2:888896/1‑139 (MQ=255)
cGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg > 1:1582917/1‑50 (MQ=255)
cGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 2:1582917/50‑1 (MQ=255)
ggtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGttt > 1:1750751/1‑69 (MQ=255)
ggtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGttt < 2:1750751/69‑1 (MQ=255)
gtgttACGAATAGTTGGGCATGCAAGGTTAGCGCCTGCGCTCCAGCCa < 1:1743177/48‑1 (MQ=255)
gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 2:948131/45‑1 (MQ=255)
gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg > 1:948131/1‑45 (MQ=255)
gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCa > 2:1743177/1‑48 (MQ=255)
gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgc > 1:199970/1‑139 (MQ=255)
gtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgc > 1:55109/1‑139 (MQ=255)
gtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgc > 2:732123/1‑139 (MQ=255)
tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCaa < 2:1347793/118‑1 (MQ=255)
tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCaa > 1:1347793/1‑118 (MQ=255)
tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc > 1:905627/1‑139 (MQ=255)
tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc > 1:728208/1‑139 (MQ=255)
tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCACTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc > 2:1206688/1‑139 (MQ=255)
tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCACTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc < 1:1206688/139‑1 (MQ=255)
aCGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgccg > 1:1340037/1‑139 (MQ=255)
aCGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGAGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgccg > 1:1053774/1‑139 (MQ=255)
gAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGAc > 2:1081296/1‑139 (MQ=255)
tAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAAt < 2:784973/139‑1 (MQ=255)
tAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAAt > 1:1621752/1‑139 (MQ=255)
gTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCa < 1:365188/139‑1 (MQ=255)
gggCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTAAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACACTCACGa > 1:1033555/1‑139 (MQ=255)
|
ACGGGTTTCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGA > NC_000913/4229630‑4229898
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
