Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
67 |
27.9 |
1239648 |
95.3% |
1181384 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,504,660 |
G→A |
P296P (CCC→CCT) |
ypdE ← |
aminopeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,504,660 | 0 | G | A | 100.0%
| 29.4
/ NA
| 10 | P296P (CCC→CCT) | ypdE | aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/5); total (5/5) |
TCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTGGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCA > NC_000913/2504541‑2504793
|
tcACGCGTAAGACTTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTa > 2:455144/1‑139 (MQ=255)
gATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTgccgc < 1:607757/139‑1 (MQ=255)
gATAAAAGTTGCTGCAGCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCAACGCCCCGCCGTCCGTgtcgc < 2:418707/139‑1 (MQ=255)
cAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATctgcc > 2:494818/1‑139 (MQ=255)
gTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAAc > 1:302056/1‑139 (MQ=255)
gcgGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGc < 2:346710/139‑1 (MQ=255)
cggcaatcggcaatCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCa > 1:494016/1‑139 (MQ=255)
tcggcaatcgATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCaa < 1:494818/139‑1 (MQ=255)
aTCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGgcggcg < 1:455144/139‑1 (MQ=255)
acTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCa > 1:522340/1‑139 (MQ=255)
|
TCACGCGTAAGACGTTGAATAAGGGCAGATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTGGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAAGTTTTGGCGGCGCAATCA > NC_000913/2504541‑2504793
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
