Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
67 |
31.4 |
1402038 |
96.1% |
1347358 |
109.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,505,003 |
T→C |
H182R (CAC→CGC) |
ypdE ← |
aminopeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,505,003 | 0 | T | C | 100.0%
| 41.3
/ NA
| 12 | H182R (CAC→CGC) | ypdE | aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/5); total (7/5) |
GCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCT > NC_000913/2504908‑2505138
|
gCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCg < 1:157363/139‑1 (MQ=255)
acCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCCAACCGAGGCGGtca > 2:454375/1‑139 (MQ=255)
gggTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCCAACCGAGGCGGTCATCAAAgg < 1:454375/139‑1 (MQ=255)
gCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCg < 1:176154/115‑1 (MQ=255)
gCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCg > 2:176154/1‑115 (MQ=255)
ccgcGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGtcatc < 1:626018/116‑1 (MQ=255)
ccgcGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGtcatc > 2:626018/1‑116 (MQ=255)
ctctTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAAcc > 1:566293/1‑139 (MQ=255)
cGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGaaa > 1:549194/1‑139 (MQ=255)
cTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTAt > 2:643631/1‑139 (MQ=255)
tCCGCAGGTAGTTCGGTGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGc > 2:637017/1‑139 (MQ=255)
ggCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTccc < 2:258210/139‑1 (MQ=255)
tcgcgcagttcgcgcagtAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCt < 2:549194/139‑1 (MQ=255)
|
GCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCT > NC_000913/2504908‑2505138
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
