Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
67 |
27.9 |
1239648 |
95.3% |
1181384 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,505,003 |
T→C |
H182R (CAC→CGC) |
ypdE ← |
aminopeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,505,003 | 0 | T | C | 100.0%
| 49.7
/ NA
| 14 | H182R (CAC→CGC) | ypdE | aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/6); total (8/6) |
TCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATT > NC_000913/2504869‑2505131
|
tCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAg > 2:457304/1‑139 (MQ=255)
cagGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCa < 1:240949/139‑1 (MQ=255)
ggTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAAc < 2:302056/139‑1 (MQ=255)
tGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATa > 1:458556/1‑139 (MQ=255)
cGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCgg < 1:302429/139‑1 (MQ=255)
cgGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAgg < 1:317324/139‑1 (MQ=255)
tGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCa > 2:502928/1‑139 (MQ=255)
acTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTg < 1:144340/120‑1 (MQ=255)
acTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTg > 2:144340/1‑120 (MQ=255)
aGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGc < 1:393106/84‑1 (MQ=255)
aGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGc > 2:393106/1‑84 (MQ=255)
ttCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATTAAACGTGACGCGATCGCCGGGACGAAtt > 2:565673/1‑139 (MQ=255)
ttCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGTCGGGACGAAtt > 2:400669/1‑139 (MQ=255)
ttCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAAtt > 1:49557/1‑139 (MQ=255)
|
TCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATT > NC_000913/2504869‑2505131
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
