Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	67	27.9	1239648	95.3%	1181384	112.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,733,223	G→A	D317D (GAC→GAT)	clpB ←	protein disaggregation chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,733,223	0	G	A	100.0%	99.9 / NA	30	D317D (GAC→GAT)	clpB	protein disaggregation chaperone
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/15); total (15/15)

GGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACG  >  NC_000913/2733090‑2733358

ggtggtGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAg                                                                                                                                    >  2:608624/1‑139 (MQ=255)

     tgCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTGATCAAGcgtcg                                                                                                                               >  2:399217/1‑139 (MQ=255)

      gCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGcgtcgt                                                                                                                              <  1:608624/139‑1 (MQ=255)

          ttCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGTTATTCATCAAGCGTCGTGGCa                                                                                                                          >  2:547269/1‑139 (MQ=255)

           tCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCAc                                                                                                                         <  1:304627/139‑1 (MQ=255)

           tCGTAACGTTCTTGCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACTACGTCCCAGCGCAGCATCTCTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCAc                                                                                                                         <  1:406342/139‑1 (MQ=255)

               aaCGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTAc                                                                                                                     >  1:399603/1‑139 (MQ=255)

                  gTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCa                                                                                                                  <  2:52596/139‑1 (MQ=255)

                            gCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACg                                                                                                                    <  2:602437/127‑1 (MQ=255)

                            gCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACg                                                                                                                    >  1:602437/1‑127 (MQ=255)

                                cGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTcaccac                                                                                                    >  2:327757/1‑139 (MQ=255)

                                               gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGtt                                                                                     >  2:532387/1‑139 (MQ=255)

                                                     tCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCa                                                                               >  2:456937/1‑139 (MQ=255)

                                                              ggCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCgg                                                                      >  2:354555/1‑139 (MQ=255)

                                                                                     gAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTa                                               <  1:547269/139‑1 (MQ=255)

                                                                                            cgTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCgcgc                                        <  1:399217/139‑1 (MQ=255)

                                                                                               tCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGa                                     <  2:554815/139‑1 (MQ=255)

                                                                                               tCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGa                                     <  1:327757/139‑1 (MQ=255)

                                                                                                      cagcaTCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATg                                >  1:434707/1‑137 (MQ=255)

                                                                                                      cagcaTCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATg                                <  2:434707/137‑1 (MQ=255)

                                                                                                         caTCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATg                           >  2:252937/1‑139 (MQ=255)

                                                                                                          aTCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGt                          <  1:252937/139‑1 (MQ=255)

                                                                                                                ttCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTg                                                             <  2:195428/98‑1 (MQ=255)

                                                                                                                ttCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTg                                                             >  1:195428/1‑98 (MQ=255)

                                                                                                                     tGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGAt               >  2:316846/1‑139 (MQ=255)

                                                                                                                      gTACTGGCGATGTTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATa              <  2:399603/139‑1 (MQ=255)

                                                                                                                            gCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCa                                                                               >  2:296164/1‑68 (MQ=255)

                                                                                                                            gCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCa                                                                               <  1:296164/68‑1 (MQ=255)

                                                                                                                                 atTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGAc   >  2:605577/1‑139 (MQ=255)

                                                                                                                                  ttCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACg  <  1:605577/139‑1 (MQ=255)

GGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGACG  >  NC_000913/2733090‑2733358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A