Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
67 |
31.4 |
1402038 |
96.1% |
1347358 |
109.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,733,223 |
G→A |
D317D (GAC→GAT) |
clpB ← |
protein disaggregation chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,733,223 | 0 | G | A | 100.0%
| 99.1
/ NA
| 29 | D317D (GAC→GAT) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/11); total (18/11) |
TGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGAC > NC_000913/2733095‑2733357
|
tgCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGcgtcg > 1:366838/1‑139 (MQ=255)
cGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCAcc > 1:265746/1‑139 (MQ=255)
aaCGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCGTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTAc > 1:155404/1‑139 (MQ=255)
aCGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACg < 2:366838/139‑1 (MQ=255)
aaTCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTgg > 2:605346/1‑110 (MQ=255)
aaTCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTgg < 1:605346/110‑1 (MQ=255)
cAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAgcgc < 1:327203/139‑1 (MQ=255)
aTCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTc < 2:120364/139‑1 (MQ=255)
cAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGtt > 2:694166/1‑139 (MQ=255)
aaGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGAGCCAGCGCCGGTTTCAGCATGTTTcc > 1:444666/1‑139 (MQ=255)
gCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAATCGTCGTGGCACCTACCCAGTGCAATTAACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGc < 2:444666/139‑1 (MQ=255)
ggCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTcc > 1:66232/1‑139 (MQ=255)
cacTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCa < 2:265746/139‑1 (MQ=255)
tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCa < 1:148861/98‑1 (MQ=255)
tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCa > 2:148861/1‑98 (MQ=255)
tCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGc > 2:411471/1‑139 (MQ=255)
cgacgTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCcg > 2:239335/1‑139 (MQ=255)
ctCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCg > 1:517128/2‑139 (MQ=255)
tCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGa > 1:35412/1‑139 (MQ=255)
agcaTCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTa < 2:155404/139‑1 (MQ=255)
tGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATtcgtcg < 2:218894/137‑1 (MQ=255)
tGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATtcgtcg > 1:218894/1‑137 (MQ=255)
tGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGAATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGAt > 2:664367/1‑139 (MQ=255)
gCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAgg > 1:291490/1‑139 (MQ=255)
atatTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTAcc > 1:649710/1‑99 (MQ=255)
atatTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTAcc < 2:649710/99‑1 (MQ=255)
atTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGttt > 1:613224/1‑58 (MQ=255)
atTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGttt < 2:613224/58‑1 (MQ=255)
atTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGAc > 1:211309/1‑139 (MQ=255)
|
TGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGAC > NC_000913/2733095‑2733357
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
