Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
67 |
31.4 |
1402038 |
96.1% |
1347358 |
109.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,542,393 |
G→A |
A40A (GCC→GCT) |
cysU ← |
sulfate/thiosulfate ABC transporter permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,542,393 | 0 | G | A | 100.0%
| 96.0
/ NA
| 28 | A40A (GCC→GCT) | cysU | sulfate/thiosulfate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/12); total (16/12) |
GCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATCAGTT > NC_000913/2542268‑2542517
|
gCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGt > 2:343079/1‑132 (MQ=255)
gCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGt < 1:343079/132‑1 (MQ=255)
tCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAg > 1:56473/1‑139 (MQ=255)
ccGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCa < 2:14703/127‑1 (MQ=255)
ccGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCa > 1:14703/1‑127 (MQ=255)
aTCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCa > 2:185568/1‑139 (MQ=255)
caAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGcac < 1:74464/134‑1 (MQ=255)
caAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGcac > 2:74464/1‑134 (MQ=255)
gCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAAcagca < 1:185568/139‑1 (MQ=255)
tAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAgg > 1:682528/1‑139 (MQ=255)
cgcgACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGagag < 2:510042/81‑1 (MQ=255)
cgcgACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGagag > 1:510042/1‑81 (MQ=255)
cgcgACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCgg < 2:291393/84‑1 (MQ=255)
cgcgACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCgg > 1:291393/1‑84 (MQ=255)
tGATCACCTCCCAGTACTGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTgga < 1:640931/139‑1 (MQ=255)
tGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGgc < 1:357440/48‑1 (MQ=255)
tGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGgc > 2:357440/1‑48 (MQ=255)
tGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCg > 1:681897/1‑87 (MQ=255)
tGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCg < 2:681897/87‑1 (MQ=255)
tGCGCCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATCa > 1:325124/1‑139 (MQ=255)
gcgcCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGgaggag < 1:628903/125‑1 (MQ=255)
gcgcCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGgaggag > 2:628903/1‑125 (MQ=255)
cgcCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCTAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCTCGCGTCTGGAGGAGACAGCAAACATCAGt > 2:472612/1‑139 (MQ=255)
cgcCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATc > 2:581419/1‑136 (MQ=255)
cgcCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATc < 1:581419/136‑1 (MQ=255)
gcCCAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATCAGtt > 2:162012/1‑139 (MQ=255)
cAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCAcg > 1:56465/1‑108 (MQ=255)
cAGCTCATCTGAGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCAcg < 2:56465/108‑1 (MQ=255)
|
GCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCATCTGGGCCAGTTGCATCACCAGCGCGGAGAGCGGCAGCAGCAAAATCAGGCACACAAACAGCAGACTGGTGCCGAGGCTTAAGGTAAAGCCCGGCAGCACGCGTCTGGAGGAGACAGCAAACATCAGTT > NC_000913/2542268‑2542517
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
