Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	31	30.9	1275452	97.8%	1247392	117.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,963,498	G→A	T73T (ACC→ACT)	ppdB ←	putative prepilin peptidase‑dependent protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,963,498	0	G	A	100.0%	110.2 / NA	32	T73T (ACC→ACT)	ppdB	putative prepilin peptidase‑dependent protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/12); total (20/12)

TGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCG  >  NC_000913/2963370‑2963632

tGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGc                                                                                                                              >  1:362632/1‑139 (MQ=255)

       tCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACa                                                                                                                       >  1:628374/1‑139 (MQ=255)

           aCGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACa                                                                                                                                   >  1:297593/1‑123 (MQ=255)

           aCGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACa                                                                                                                                   <  2:297593/123‑1 (MQ=255)

                  ccAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGccc                                                                                                            <  1:628479/139‑1 (MQ=255)

                                   ttttACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTcgcg                                                                                           <  2:628374/139‑1 (MQ=255)

                                     ttACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGAc                                                                                         <  2:255092/139‑1 (MQ=255)

                                             tCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATa                                                                                 >  2:89047/1‑139 (MQ=255)

                                                             cGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTc                                                                 <  2:345335/139‑1 (MQ=255)

                                                             cGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTc                                                                 >  2:335291/1‑139 (MQ=255)

                                                             cGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTc                                                                 >  1:345335/1‑139 (MQ=255)

                                                               ttACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGa                                                                                          >  2:492588/1‑112 (MQ=255)

                                                               ttACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGa                                                                                          <  1:492588/112‑1 (MQ=255)

                                                               ttACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCAt                                                               >  1:152393/1‑139 (MQ=255)

                                                                     ttCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCa                                                         >  2:169982/1‑139 (MQ=255)

                                                                     ttCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCa                                                         >  2:346747/1‑139 (MQ=255)

                                                                       cGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCATCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAgc                                                       >  1:519846/1‑139 (MQ=255)

                                                                       cGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAAcc                                                                                                                 >  1:581327/1‑81 (MQ=255)

                                                                       cGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAAcc                                                                                                                 <  2:581327/81‑1 (MQ=255)

                                                                         cATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAgcgc                                                     <  2:519846/139‑1 (MQ=255)

                                                                          aTCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAgcgc                                                     >  2:528179/1‑138 (MQ=255)

                                                                          aTCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAgcgc                                                     <  1:528179/138‑1 (MQ=255)

                                                                               aCTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCtt                                               >  2:216949/1‑139 (MQ=255)

                                                                                 tGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTAc                                             >  2:29924/1‑139 (MQ=255)

                                                                                      aaTGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTg                                        <  1:335291/139‑1 (MQ=255)

                                                                                                           gACAATTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGt                   >  2:183786/1‑139 (MQ=255)

                                                                                                               aTTTCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACg               >  2:582696/1‑139 (MQ=255)

                                                                                                                tttCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACgc              <  1:346747/139‑1 (MQ=255)

                                                                                                                  tCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCg                                                                             >  1:603737/1‑74 (MQ=255)

                                                                                                                  tCCAGCCCTTCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCg                                                                             <  2:603737/74‑1 (MQ=255)

                                                                                                                           tCGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGc   >  1:596149/1‑139 (MQ=255)

                                                                                                                            cGCCAGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCg  >  2:1742/1‑139 (MQ=255)

TGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGTTACTGTTCGCATCCCACTGCACAATGACACAGTCACCCTGTCCGACAATTTCCAGCCCTTCGCCGGTACAGATGCCATGACAATAACCCGCCCTCTGGAGATGCTTCGCGACGGTAAATACCCGCAGCCAGATTTCATCTTCCAGCGCCAGCTTACGGGTGCTCGTTAAACTTTCACGCTGTAACGCAGGCAGAAAGCG  >  NC_000913/2963370‑2963632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A