Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 69 53.0 2252012 96.1% 2164183 115.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,965,748 T→C D97G (GAC→GGC)  lgt ← phosphatidylglycerol‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,965,7480TC100.0% 174.0 / NA 49D97G (GAC→GGC) lgtphosphatidylglycerol‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/28);  total (21/28)

ACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTG  >  NC_000913/2965618‑2965879
                                                                                                                                  |                                                                                                                                   
aCCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACGACGATAACGGCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGaca                                                                                                                             <  2:692978/139‑1 (MQ=255)
 ccAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGacac                                                                                                                            <  1:93171/139‑1 (MQ=255)
   aaGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACgg                                                                                                                          <  2:325662/139‑1 (MQ=255)
                 tGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGTCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCgg                                                                                                            >  2:74468/1‑139 (MQ=255)
                 tGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCgg                                                                                                            >  1:64924/1‑139 (MQ=255)
                       gtgCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCAAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACCGAACAGATACACCGGATCggt                                                                                                      >  2:657092/1‑138 (MQ=255)
                         gCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCa                                                                                                    >  2:996950/1‑139 (MQ=255)
                         gCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCa                                                                                                    >  2:134917/1‑139 (MQ=255)
                          cGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCAt                                                                                                   >  2:405936/1‑139 (MQ=255)
                             tAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                                                                >  2:337339/1‑139 (MQ=255)
                                     gagaCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGTCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGa                                                                                        <  1:74468/139‑1 (MQ=255)
                                      agaCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGaa                                                                                       >  2:687081/1‑139 (MQ=255)
                                       gaCCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGaaa                                                                                      <  1:337339/139‑1 (MQ=255)
                                           tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                                                                >  2:946341/1‑125 (MQ=255)
                                           tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATaaa                                                                                                <  1:946341/125‑1 (MQ=255)
                                           tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGt                                                                                  >  1:124917/1‑139 (MQ=255)
                                           tGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGt                                                                                  >  1:514152/1‑139 (MQ=255)
                                            ggAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGa                                                                                        >  2:533542/1‑132 (MQ=255)
                                            ggAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGa                                                                                        <  1:533542/132‑1 (MQ=255)
                                               agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaa                                                                              <  2:514152/139‑1 (MQ=255)
                                                gaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                                                             <  1:62122/139‑1 (MQ=255)
                                                    gAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaa                                                                         <  1:990710/139‑1 (MQ=255)
                                                       cGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACAt                                                                      <  1:687081/139‑1 (MQ=255)
                                                           tAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAAcc                                                                  <  2:181969/139‑1 (MQ=255)
                                                           tAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAAcc                                                                  <  1:405936/139‑1 (MQ=255)
                                                                 ggcgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACg                                                            <  1:499963/139‑1 (MQ=255)
                                                                   cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGacac                                                                                                                            <  1:140035/73‑1 (MQ=255)
                                                                   cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGacac                                                                                                                            >  2:140035/1‑73 (MQ=255)
                                                                   cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                                                             <  2:769702/120‑1 (MQ=255)
                                                                   cgggcgAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaac                                                                             >  1:769702/1‑120 (MQ=255)
                                                                           gATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAgg                                                  <  2:64924/139‑1 (MQ=255)
                                                                                 catcaCCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACg                                            <  2:1096310/139‑1 (MQ=255)
                                                                                      ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGt                                                           <  2:65464/119‑1 (MQ=255)
                                                                                      ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGt                                                           >  1:65464/1‑119 (MQ=255)
                                                                                      ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGa                                              >  2:447950/1‑132 (MQ=255)
                                                                                      ccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGa                                              <  1:447950/132‑1 (MQ=255)
                                                                                        acGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGa                                     <  1:522750/139‑1 (MQ=255)
                                                                                          gATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAg                                   >  1:822368/1‑139 (MQ=255)
                                                                                              aCGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg                               <  2:329691/139‑1 (MQ=255)
                                                                                               cGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGc                              <  2:1047176/139‑1 (MQ=255)
                                                                                                gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCa                             <  2:305118/139‑1 (MQ=255)
                                                                                                gCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCa                             <  1:49046/139‑1 (MQ=255)
                                                                                                 ccAATCAGGCCGCCGTGGAAAGTCATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                                                                        <  1:952171/95‑1 (MQ=255)
                                                                                                 ccAATCAGGCCGCCGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTagaacagaac                                                                        >  2:952171/1‑95 (MQ=255)
                                                                                                         gccgccGTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAg                    >  1:274132/1‑139 (MQ=255)
                                                                                                            gccgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGttt                 >  2:1028489/1‑139 (MQ=255)
                                                                                                              cgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGa                                     >  1:812396/1‑117 (MQ=255)
                                                                                                              cgTGGAAAGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGa                                     <  2:812396/117‑1 (MQ=255)
                                                                                                                    aaGACATGCCGCCGCCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTc         <  1:996950/139‑1 (MQ=255)
                                                                                                                           gccgccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg                               <  1:109775/110‑1 (MQ=255)
                                                                                                                           gccgccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg                               >  2:109775/1‑110 (MQ=255)
                                                                                                                           gccgccgccCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTg  >  2:989902/1‑139 (MQ=255)
                                                                                                                             cgccgcCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGc                                  <  1:154964/105‑1 (MQ=255)
                                                                                                                             cgccgcCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGc                                  >  2:154964/1‑105 (MQ=255)
                                                                                                                                  |                                                                                                                                   
ACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTG  >  NC_000913/2965618‑2965879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A