Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 63 64.8 2934156 93.7% 2749304 111.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,400,093 G→A A332V (GCG→GTG)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,400,0930GA100.0% 205.2 / NA 59A332V (GCG→GTG) mreBcell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/26);  total (33/26)

CTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCG  >  NC_000913/3399962‑3400227
                                                                                                                                   |                                                                                                                                      
cTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTgcc                                                                                                                                 >  2:1008397/1‑139 (MQ=255)
   aaaaTTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTgccgcc                                                                                                                              >  2:436892/1‑139 (MQ=255)
         ggCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                        >  2:281210/1‑139 (MQ=255)
         ggCGTCATAAGATATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                        <  1:950988/139‑1 (MQ=255)
          gCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcgc                                                                                                                       >  2:408780/1‑139 (MQ=255)
           cTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcgcg                                                                                                                      <  2:1388662/139‑1 (MQ=255)
                       tGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCa                                                                                                          >  1:54011/1‑139 (MQ=255)
                         cGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGc                                                                                                        <  1:741981/139‑1 (MQ=255)
                         cGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGc                                                                                                        >  1:499239/1‑139 (MQ=255)
                                     tCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGca                                                                                            <  2:1097221/139‑1 (MQ=255)
                                     tCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTACATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGca                                                                                            <  2:364173/139‑1 (MQ=255)
                                        gACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaaca                                                                                         >  2:708981/1‑139 (MQ=255)
                                         aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaacaa                                                                                        >  2:1378821/1‑139 (MQ=255)
                                         aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaacaa                                                                                        >  1:1096770/1‑139 (MQ=255)
                                         aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaacaa                                                                                        >  1:962320/1‑139 (MQ=255)
                                                  ggTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGCCTGGaa                                                                               >  1:204/1‑139 (MQ=255)
                                                  ggTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGaa                                                                               >  2:78939/1‑139 (MQ=255)
                                                   gTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAAt                                                                              >  2:100146/1‑139 (MQ=255)
                                                    tAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATg                                                                             <  1:78939/139‑1 (MQ=255)
                                                       aCAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCg                                                                          <  2:1105346/139‑1 (MQ=255)
                                                            cacTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGTCTGGAATGCCGGTttc                                                                     >  1:928875/1‑139 (MQ=255)
                                                             acTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttct                                                                    >  1:160310/1‑139 (MQ=255)
                                                               ttCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttcttc                                                                  <  1:436892/139‑1 (MQ=255)
                                                               ttCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGCTttcttc                                                                  <  2:962320/139‑1 (MQ=255)
                                                                     tgcctgcATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTaa                                                            >  1:1250271/1‑139 (MQ=255)
                                                                       cctgcATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGAc                                                                                    >  2:523714/1‑113 (MQ=255)
                                                                       cctgcATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACa                                                          <  1:1378821/139‑1 (MQ=255)
                                                                       cctgcATCCGATGACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGAc                                                                                    <  1:523714/113‑1 (MQ=255)
                                                                        ctgcATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACaa                                                         <  2:499239/139‑1 (MQ=255)
                                                                           cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                        >  2:1230485/1‑73 (MQ=255)
                                                                           cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                        <  1:1230485/73‑1 (MQ=255)
                                                                           cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTgg                                                                                 >  1:153941/1‑112 (MQ=255)
                                                                           cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTgg                                                                                 <  2:153941/112‑1 (MQ=255)
                                                                            aTCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACgg                                                     >  2:192161/1‑139 (MQ=255)
                                                                            aTCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACgg                                                     >  2:381089/1‑139 (MQ=255)
                                                                             tCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGt                                                    <  1:12291/139‑1 (MQ=255)
                                                                               cGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCa                                                  <  2:54011/139‑1 (MQ=255)
                                                                                 aTTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGc                                                                                                        <  2:1132293/83‑1 (MQ=255)
                                                                                 aTTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGc                                                                                                        >  1:1132293/1‑83 (MQ=255)
                                                                                  ttACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAgg                                               <  1:192161/139‑1 (MQ=255)
                                                                                  ttACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAgg                                               <  1:281210/139‑1 (MQ=255)
                                                                                          cGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACgcag                                       >  2:53416/1‑139 (MQ=255)
                                                                                          cGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACgcag                                       <  2:1096770/139‑1 (MQ=255)
                                                                                            cTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                   <  2:40557/77‑1 (MQ=255)
                                                                                            cTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                   >  1:40557/1‑77 (MQ=255)
                                                                                             tGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGcagcag                                    <  2:250532/139‑1 (MQ=255)
                                                                                                aCAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                        <  2:146163/52‑1 (MQ=255)
                                                                                                aCAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                        >  1:146163/1‑52 (MQ=255)
                                                                                                    gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGt                                                                                                            <  2:154805/60‑1 (MQ=255)
                                                                                                    gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGt                                                                                                            >  1:154805/1‑60 (MQ=255)
                                                                                                    gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGcca                             >  2:342355/1‑139 (MQ=255)
                                                                                                    gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGcca                             >  2:457526/1‑139 (MQ=255)
                                                                                                     tCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGccac                            >  2:676105/1‑139 (MQ=255)
                                                                                                         cgccgTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGccacaccg                        >  1:1102889/1‑135 (MQ=255)
                                                                                                                cATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCAGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGc                 >  2:849175/1‑139 (MQ=255)
                                                                                                                       aTCATTTCCAGCACTTTGCCGCCACCGCGCGCcaca                                                                                                                 <  1:700655/36‑1 (MQ=255)
                                                                                                                       aTCATTTCCAGCACTTTGCCGCCACCGCGCGCcaca                                                                                                                 >  2:700655/1‑36 (MQ=255)
                                                                                                                             tCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGGGCCACCACCGGTGAGCACCATGCCGCGCt    >  1:31913/1‑139 (MQ=255)
                                                                                                                               cAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCg  <  1:342355/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
CTAAAAATTGGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCG  >  NC_000913/3399962‑3400227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A