Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 63 64.8 2934156 93.7% 2749304 111.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,281,472 A→G E498G (GAG→GGG)  yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,281,4720AG100.0% 224.8 / NA 59E498G (GAG→GGG) yjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/25);  total (34/25)

CGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGT  >  NC_000913/4281345‑4281606
                                                                                                                               |                                                                                                                                      
cGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGc                                                                                                                             >  2:1368739/1‑139 (MQ=255)
 gTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCg                                                                                                                            <  1:703063/139‑1 (MQ=255)
          cggATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCg                                                                                                                   >  2:1137450/1‑139 (MQ=255)
           ggATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGt                                                                                                                  >  2:1460857/1‑139 (MQ=255)
              tACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTg                                                                                                               <  1:1368739/139‑1 (MQ=255)
                      agaATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                       >  1:385951/1‑139 (MQ=255)
                       gaATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTg                                                                                                      <  1:43718/139‑1 (MQ=255)
                           atCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGtt                                                                                                  >  1:655785/1‑139 (MQ=255)
                                    cAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                       <  2:293219/125‑1 (MQ=255)
                                    cAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                       >  1:293219/1‑125 (MQ=255)
                                        tgctTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGa                                                                                     >  2:985219/1‑139 (MQ=255)
                                         gctTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGaa                                                                                    >  2:1112162/1‑139 (MQ=255)
                                               ggAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCa                                                                                                         <  2:538783/112‑1 (MQ=255)
                                               ggAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCa                                                                                                         >  1:538783/1‑112 (MQ=255)
                                                   gTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTaccacc                                                                          >  2:1160441/1‑139 (MQ=255)
                                                    tCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGa                                                                                              >  1:1464677/1‑118 (MQ=255)
                                                    tCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGa                                                                                              <  2:1464677/118‑1 (MQ=255)
                                                       gTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTGCCACCTgcg                                                                      <  1:985219/139‑1 (MQ=255)
                                                         tctcGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCg                                                                                                                            >  1:52093/1‑83 (MQ=255)
                                                         tctcGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCg                                                                                                                            <  2:52093/83‑1 (MQ=255)
                                                            cgcgTCATTGGTAACCTGCGTCGTCGCGGCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCca                                                                 <  1:423561/139‑1 (MQ=255)
                                                             gcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCcac                                                                >  1:916518/1‑139 (MQ=255)
                                                               gTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCCTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                              <  1:1382014/139‑1 (MQ=255)
                                                                 taTTTGTAACCTTCGTTGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAAGAAGGGAACCTTGAGCGTCGCTTCCGGCCTGCCGGATTGAGTTCTGAACGTGCTGGACTTTACCCCCCGCGCGCCACACgg                                                            <  2:904635/138‑1 (MQ=255)
                                                                  aTTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                       >  1:59108/1‑95 (MQ=255)
                                                                  aTTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                       <  2:59108/95‑1 (MQ=255)
                                                                       tAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATc                                                      >  1:716511/1‑139 (MQ=255)
                                                                           cTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGt                                                                                                                           <  1:311579/66‑1 (MQ=255)
                                                                           cTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGt                                                                                                                           >  2:311579/1‑66 (MQ=255)
                                                                             gCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAAc                                                >  1:102653/1‑139 (MQ=255)
                                                                              cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTga                                                                                        >  2:878773/1‑97 (MQ=255)
                                                                              cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGc                                                                                        <  1:878773/98‑1 (MQ=255)
                                                                              cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                               >  2:1050228/1‑139 (MQ=255)
                                                                              cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                               >  1:80772/1‑139 (MQ=255)
                                                                              cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                               >  2:312517/1‑139 (MQ=255)
                                                                              cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                               >  2:1037085/1‑139 (MQ=255)
                                                                               gtcgtcGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGcc                                                                  >  2:882125/1‑119 (MQ=255)
                                                                               gtcgtcGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGcc                                                                  <  1:882125/119‑1 (MQ=255)
                                                                                tcgtcgCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCg                                                                                                                   >  2:907830/1‑69 (MQ=255)
                                                                                tcgtcgCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCg                                                                                                                   <  1:907830/69‑1 (MQ=255)
                                                                                 cgtcgCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcg                                                                      >  2:394666/1‑113 (MQ=255)
                                                                                 cgtcgCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcg                                                                      <  1:394666/113‑1 (MQ=255)
                                                                                     gcgcCGATGGCCGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAATGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCt                                        >  2:1067368/1‑139 (MQ=255)
                                                                                      cgcCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTg                                       >  1:286573/1‑139 (MQ=255)
                                                                                        ccGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                              <  1:287714/114‑1 (MQ=255)
                                                                                        ccGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                              >  2:287714/1‑114 (MQ=255)
                                                                                          gATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCaaa                                   <  1:442061/139‑1 (MQ=255)
                                                                                               aCGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTa                              <  1:1160441/139‑1 (MQ=255)
                                                                                                    acgacgTAGAAAGTTCCGTGCAGGAAGGGAACCGTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTGCCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTTCa                         <  2:1078079/139‑1 (MQ=255)
                                                                                                        cgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGAt                     >  2:377908/1‑139 (MQ=255)
                                                                                                          ttGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                              <  1:1223357/96‑1 (MQ=255)
                                                                                                          ttGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                              >  2:1223357/1‑96 (MQ=255)
                                                                                                           tGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                       >  2:225499/1‑54 (MQ=255)
                                                                                                           tGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt                                                                                                       <  1:225499/54‑1 (MQ=255)
                                                                                                                      tGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGa       >  2:32419/1‑139 (MQ=255)
                                                                                                                      tGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGa       >  1:148129/1‑139 (MQ=255)
                                                                                                                       gCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGaa      <  2:148129/139‑1 (MQ=255)
                                                                                                                       gCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGaa      <  2:80772/139‑1 (MQ=255)
                                                                                                                       gCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGaa      <  1:101786/139‑1 (MQ=255)
                                                                                                                           gAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGt  >  2:1103890/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                      
CGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGT  >  NC_000913/4281345‑4281606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A