Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	59	52.6	2411032	94.1%	2268781	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,472	A→G	E498G (GAG→GGG)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,472	0	A	G	100.0%	154.0 / NA	41	E498G (GAG→GGG)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/19); total (22/19)

AGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTG  >  NC_000913/4281340‑4281600

aggagCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCt                                                                                                                            >  2:338548/1‑139 (MQ=255)

aggagCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGT‑ACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCtt                                                                                                                           >  2:678155/1‑139 (MQ=255)

 ggagCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCtt                                                                                                                           >  1:387395/1‑139 (MQ=255)

    gCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAg                                                                                                                        <  2:491105/139‑1 (MQ=255)

            cggcggATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCtt                                                                                                                <  1:811822/139‑1 (MQ=255)

                 gATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTc                                                                                                           >  1:1017037/1‑139 (MQ=255)

                            gaATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAg                                                                                                                        <  2:542056/115‑1 (MQ=255)

                            gaATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAg                                                                                                                        >  1:542056/1‑115 (MQ=255)

                                     tAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGaa                                                                                       <  2:940552/139‑1 (MQ=255)

                                     tAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCATCCGTCTGGCGGCATTGCGTTCTGaa                                                                                       >  2:770271/1‑139 (MQ=255)

                                       aCCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACg                                                                                     >  1:1114025/1‑139 (MQ=255)

                                           gctgctTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAt                                                                                                  <  2:473273/122‑1 (MQ=255)

                                           gctgctTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAt                                                                                                  >  1:473273/1‑122 (MQ=255)

                                            ctgctTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTg                                                                                >  1:418204/1‑139 (MQ=255)

                                            ctgctTACGGAGGTCAGTTCTCGCGTCAGTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGACTGGCGGCATTGCGTTCTGAACGTGCTg                                                                                >  1:750094/1‑139 (MQ=255)

                                                           aGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgc                                                                 >  2:480921/1‑139 (MQ=255)

                                                           aGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgc                                                                 >  2:87041/1‑139 (MQ=255)

                                                           aGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgc                                                                 >  1:974070/1‑139 (MQ=255)

                                                                 cgcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCca                                                           <  1:612989/139‑1 (MQ=255)

                                                                    gTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                        <  2:750094/139‑1 (MQ=255)

                                                                    gTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                        <  1:1077599/139‑1 (MQ=255)

                                                                    gTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCacac                                                        <  1:326134/139‑1 (MQ=255)

                                                                       aTTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACggg                                                     <  1:1007825/139‑1 (MQ=255)

                                                                                  gCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAAc                                          >  2:292043/1‑139 (MQ=255)

                                                                                   cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                         >  1:924573/1‑139 (MQ=255)

                                                                                   cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACg                                         >  2:299615/1‑139 (MQ=255)

                                                                                        tcgCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTgg                                                                                                        <  2:328934/71‑1 (MQ=255)

                                                                                        tcgCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTgg                                                                                                        >  1:328934/1‑71 (MQ=255)

                                                                                               gATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCaaa                             >  1:829683/1‑139 (MQ=255)

                                                                                               gATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCaaa                             <  2:418204/139‑1 (MQ=255)

                                                                                               gATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCaaa                             <  2:132028/139‑1 (MQ=255)

                                                                                                aTGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGTCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGaaa                                      <  2:787349/129‑1 (MQ=255)

                                                                                                aTGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGaaa                                      >  1:787349/1‑129 (MQ=255)

                                                                                                        aaCGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGc                    >  1:1157769/1‑139 (MQ=255)

                                                                                                         acgacgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCa                   <  2:829683/139‑1 (MQ=255)

                                                                                                             cgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTgg                                                                                                        >  1:161591/1‑50 (MQ=255)

                                                                                                             cgTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTgg                                                                                                        <  2:161591/50‑1 (MQ=255)

                                                                                                                    aGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGAtt        <  2:974070/139‑1 (MQ=255)

                                                                                                                        ccGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCt    >  1:1034424/1‑139 (MQ=255)

                                                                                                                          gTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGATCTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTg  <  2:864388/139‑1 (MQ=255)

                                                                                                                          gTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTg  <  2:1017037/139‑1 (MQ=255)

AGGAGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTG  >  NC_000913/4281340‑4281600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A