Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	63	64.8	2934156	93.7%	2749304	111.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,345,727	A→G	F534L (TTC→CTC)	fumB ←	anaerobic class I fumarate hydratase (fumarase B)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,345,727	0	A	G	100.0%	171.1 / NA	45	F534L (TTC→CTC)	fumB	anaerobic class I fumarate hydratase (fumarase B)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/19); total (26/19)

TTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTAC  >  NC_000913/4345592‑4345856

ttCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTc                                                                                                                                >  1:782110/1‑139 (MQ=255)

          aGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGt                                                                                                                              <  2:1369633/131‑1 (MQ=255)

          aGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGt                                                                                                                              >  1:1369633/1‑131 (MQ=255)

          aGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACTTTTg                                                                                                                      >  2:541192/1‑139 (MQ=255)

          aGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTg                                                                                                                      >  2:1381666/1‑139 (MQ=255)

              tGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCAt                                                                                                                  <  2:387244/139‑1 (MQ=255)

               gAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATc                                                                                                                 <  1:1381666/139‑1 (MQ=255)

                  aTTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGAc                                                                                                              <  1:1262103/139‑1 (MQ=255)

                   ttACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGAcc                                                                                                             <  1:431473/139‑1 (MQ=255)

                           gCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATaaa                                                                                                     >  1:802863/1‑139 (MQ=255)

                                                aTCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCggg                                                                                              <  2:244505/125‑1 (MQ=255)

                                                aTCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCggg                                                                                              >  1:244505/1‑125 (MQ=255)

                                                       ggCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTc                                                                         >  2:877654/1‑139 (MQ=255)

                                                          agcaTGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCa                                                                                                                   >  2:1067646/1‑94 (MQ=255)

                                                          agcaTGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCa                                                                                                                   <  1:1067646/94‑1 (MQ=255)

                                                          agcaTGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAg                                                                      >  1:447681/1‑139 (MQ=255)

                                                          agcaTGCTGCCAGGCGCTCGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAg                                                                      >  2:824439/1‑139 (MQ=255)

                                                                      ggCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCa                                                            >  1:692003/1‑137 (MQ=255)

                                                                      ggCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCa                                                            <  2:692003/137‑1 (MQ=255)

                                                                          cTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGa                                                                                                               >  2:392956/1‑82 (MQ=255)

                                                                          cTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGa                                                                                                               <  1:392956/82‑1 (MQ=255)

                                                                            gggCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGc                                                    >  1:975285/1‑139 (MQ=255)

                                                                             ggCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCt                                                   >  1:53884/1‑139 (MQ=255)

                                                                                         tACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGAt                                                                    >  1:358543/1‑110 (MQ=255)

                                                                                         tACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGAt                                                                    <  2:358543/110‑1 (MQ=255)

                                                                                                tGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGc                                                                 >  2:1221660/1‑106 (MQ=255)

                                                                                                tGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGc                                                                 <  1:1221660/106‑1 (MQ=255)

                                                                                                     ttCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATa                                                                   >  1:1119245/1‑99 (MQ=255)

                                                                                                     ttCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATa                                                                   <  2:1119245/99‑1 (MQ=255)

                                                                                                     ttCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAg                                                                  <  1:1404488/100‑1 (MQ=255)

                                                                                                     ttCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAg                                                                  >  2:1404488/1‑100 (MQ=255)

                                                                                                     ttCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGAt                           >  1:1191026/1‑139 (MQ=255)

                                                                                                      tCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAg                                                                  >  2:635874/1‑99 (MQ=255)

                                                                                                      tCGCGCACTGTTTGTTGACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAg                                                                  <  1:635874/99‑1 (MQ=255)

                                                                                                                   gttgACGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTctgc             <  2:53884/139‑1 (MQ=255)

                                                                                                                       aCGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTgcg         <  2:447681/139‑1 (MQ=255)

                                                                                                                        cGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACg                                     >  2:938118/1‑110 (MQ=255)

                                                                                                                        cGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACg                                     <  1:938118/110‑1 (MQ=255)

                                                                                                                        cGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTgcgc        >  2:1456867/1‑139 (MQ=255)

                                                                                                                        cGATTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTgcgc        >  1:13865/1‑139 (MQ=255)

                                                                                                                          aTTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCa      >  1:948163/1‑139 (MQ=255)

                                                                                                                          aTTTGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCa      >  1:1050512/1‑139 (MQ=255)

                                                                                                                            ttGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAg                                                                  <  2:285537/77‑1 (MQ=255)

                                                                                                                            ttGCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAg                                                                  >  1:285537/1‑77 (MQ=255)

                                                                                                                              gCTGGAAGAGGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGTTTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTAc  <  2:1050512/139‑1 (MQ=255)

TTCACCAGCAAGCGTGAAATTACAATCGCAAAAGAGGTGGCCAGGGGGATCACCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTAC  >  NC_000913/4345592‑4345856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A