Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 70 59.9 2639336 94.5% 2494172 113.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,554,117 A→G V518A (GTC→GCC)  maeA ← malate dehydrogenase, decarboxylating, NAD‑requiring, malic enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,554,1170AG100.0% 194.5 / NA 52V518A (GTC→GCC) maeAmalate dehydrogenase, decarboxylating, NAD‑requiring, malic enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/30);  total (22/30)

GTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAGCAAT  >  NC_000913/1553982‑1554247
                                                                                                                                       |                                                                                                                                  
gTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCtt                                                                                                                                 >  1:1087724/1‑139 (MQ=255)
 tACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCttt                                                                                                                                <  1:658683/139‑1 (MQ=255)
   cggcggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCt                                                                                                                                  >  2:1039821/1‑135 (MQ=255)
      cggTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGaa                                                                                                                           <  1:25429/139‑1 (MQ=255)
                      cGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCgg                                                                                                           <  1:239199/139‑1 (MQ=255)
                       ggCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGc                                                                                                          <  2:103541/139‑1 (MQ=255)
                        gCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCa                                                                                                         <  2:778693/139‑1 (MQ=255)
                            gCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCt                                                                                                                              <  1:3110/114‑1 (MQ=255)
                            gCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCt                                                                                                                              >  2:3110/1‑114 (MQ=255)
                            gCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTAc                                                                                                     >  1:915397/1‑139 (MQ=255)
                            gCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTAc                                                                                                     >  1:302955/1‑139 (MQ=255)
                              cAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCa                                                                                                   >  1:989801/1‑139 (MQ=255)
                                       tCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGc                                                                                          >  2:573867/1‑139 (MQ=255)
                                           cAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTACCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGtt                                                                                      >  1:532285/1‑139 (MQ=255)
                                            aaTGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTc                                                                                     <  2:674734/139‑1 (MQ=255)
                                             aTGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGa                                                                                                                            <  2:398416/99‑1 (MQ=255)
                                             aTGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGa                                                                                                                            >  1:398416/1‑99 (MQ=255)
                                                 ccTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCAc                                                                                <  1:573867/139‑1 (MQ=255)
                                                 ccTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCAc                                                                                <  2:36051/139‑1 (MQ=255)
                                                   tGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCa                                                                              >  2:309864/1‑139 (MQ=255)
                                                   tGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCa                                                                              <  2:655016/139‑1 (MQ=255)
                                                     ttGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCt                                                                                                                              >  1:329199/1‑89 (MQ=255)
                                                     ttGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCt                                                                                                                              <  2:329199/89‑1 (MQ=255)
                                                         aGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTg                                                                        <  1:1070655/139‑1 (MQ=255)
                                                           ggCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCAc                                                                                <  1:531032/129‑1 (MQ=255)
                                                           ggCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCAc                                                                                >  2:531032/1‑129 (MQ=255)
                                                               tCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTAc                                                                                                     <  1:17628/104‑1 (MQ=255)
                                                               tCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTAc                                                                                                     >  2:17628/1‑104 (MQ=255)
                                                               tCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACt                                                                  >  1:630883/1‑139 (MQ=255)
                                                                cGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCa                                                                              >  2:1001062/1‑126 (MQ=255)
                                                                cGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCa                                                                              <  1:1001062/126‑1 (MQ=255)
                                                                   cAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTgcgc                                                              <  2:483459/139‑1 (MQ=255)
                                                                            tCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTc                                                     >  1:427671/1‑139 (MQ=255)
                                                                                  ccACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCg                                                                                           <  2:118103/95‑1 (MQ=255)
                                                                                  ccACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCg                                                                                           >  1:118103/1‑95 (MQ=255)
                                                                                   cACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGcc                                              <  2:630883/139‑1 (MQ=255)
                                                                                          tgctgctgcGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGt                                                                         >  1:1079872/1‑105 (MQ=255)
                                                                                          tgctgctgcGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGt                                                                         <  2:1079872/105‑1 (MQ=255)
                                                                                              gctgcGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGctt                                   <  1:583112/139‑3 (MQ=255)
                                                                                                  cgcCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCa                               <  2:1290036/139‑1 (MQ=255)
                                                                                                  cgcCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCa                               >  1:1290036/1‑139 (MQ=255)
                                                                                                   gcCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCAt                              <  1:309864/139‑1 (MQ=255)
                                                                                                    ccATTTTGCCAACCGCAAACGTAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCg                                             <  1:840656/123‑1 (MQ=255)
                                                                                                    ccATTTTGCCAACCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCg                                             >  2:840656/1‑123 (MQ=255)
                                                                                                               aCCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTcc                                                                                                             <  1:1084266/48‑1 (MQ=255)
                                                                                                               aCCGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTcc                                                                                                             >  2:1084266/1‑48 (MQ=255)
                                                                                                                 cGCAAACGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGAc                <  2:427671/139‑1 (MQ=255)
                                                                                                                     aaCGCAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACgcgc            <  2:302955/139‑1 (MQ=255)
                                                                                                                         cAATTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGa        <  2:438291/139‑1 (MQ=255)
                                                                                                                           aTTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCgg                                                                                                           >  2:466225/1‑38 (MQ=255)
                                                                                                                           aTTGCGCGGGAGGCTTTCTGAATATCTTTCAGTTCCgg                                                                                                           <  1:466225/38‑1 (MQ=255)
                                                                                                                               cgcgGGAGGCTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAGCAAt  <  1:448626/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
GTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTTGCAGGGCTTCGGCAGAGGTTTTCACCGCCACGCCTTGCTGCTGCGCCATTTTGCCAACCGCAAACGCAATTGCGCGGGAGACTTTCTGAATATCTTTCAGTTCCGGCAGTACCATACCTTCGCCGTTCAGCACCAATGGTGAATACTGCGCCAGCGTTTCACTTGCCGACATCAGCATCTCATCGGTGATACGTGACGCGCCGGAAGCAAT  >  NC_000913/1553982‑1554247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A