Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 70 59.9 2639336 94.5% 2494172 113.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,756,495 (A)5→4 coding (798/1413 nt) pykF → pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,756,4910A.100.0% 128.4 / NA 32coding (794/1413 nt)pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (15/17);  total (15/17)

AACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAG  >  NC_000913/1756364‑1756622
                                                                                                                               |                                                                                                                                   
aaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCg                                                                                                                         <  1:128558/139‑1 (MQ=255)
 aCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGt                                                                                                                        <  1:478013/139‑1 (MQ=255)
          gCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTaaa                                                                                                               <  2:553664/139‑1 (MQ=255)
                               tCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTAt                                                                                                       <  1:766684/126‑1 (MQ=255)
                               tCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTAt                                                                                                       >  2:766684/1‑126 (MQ=255)
                               tCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAg                                                                                          <  2:886915/139‑1 (MQ=255)
                               tCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAg                                                                                          <  2:886914/139‑1 (MQ=255)
                                 cTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTa                                                                                                                 >  1:1135632/1‑114 (MQ=255)
                                 cTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTa                                                                                                                 <  2:1135632/114‑1 (MQ=255)
                                  tCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATg                                                                                       <  2:924223/139‑1 (MQ=255)
                                             gACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCAt                                                                            <  1:477875/139‑1 (MQ=255)
                                                  catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCa                                                                       >  1:1199465/1‑139 (MQ=255)
                                                     catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaaaa                                                                    >  2:1240703/1‑139 (MQ=255)
                                                             cgcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGc                                                            >  1:1177138/1‑139 (MQ=255)
                                                               cgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGccc                                                          >  1:606477/1‑139 (MQ=255)
                                                               cgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGccc                                                          <  2:1199465/139‑1 (MQ=255)
                                                                         tGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGGTGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                <  1:606325/139‑1 (MQ=255)
                                                                                     tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                    >  2:427316/1‑139 (MQ=255)
                                                                                     tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                    >  1:1145173/1‑139 (MQ=255)
                                                                                     tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                    >  2:110685/1‑139 (MQ=255)
                                                                                      cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTAt                                                                                                       <  2:1252021/71‑1 (MQ=255)
                                                                                      cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTAt                                                                                                       >  1:1252021/1‑71 (MQ=255)
                                                                                       ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                  >  2:776272/1‑139 (MQ=255)
                                                                                         ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                >  1:916018/1‑139 (MQ=255)
                                                                                                gaagTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                         >  1:1254792/1‑139 (MQ=255)
                                                                                                            gCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAg                                               >  2:1317820/1‑105 (MQ=255)
                                                                                                            gCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAg                                               <  1:1317820/105‑1 (MQ=255)
                                                                                                            gCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg             >  1:875360/1‑139 (MQ=255)
                                                                                                            gCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACgg             <  1:427316/139‑1 (MQ=255)
                                                                                                                  aagaTGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa       <  2:1268370/139‑1 (MQ=255)
                                                                                                                      tgatgatCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCa   <  2:490734/139‑1 (MQ=255)
                                                                                                                       gatgatCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg  <  1:776272/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                   
AACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAG  >  NC_000913/1756364‑1756622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A