Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 71 51.6 2219666 95.9% 2128659 118.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,756,495 (A)5→4 coding (798/1413 nt) pykF → pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,756,4910A.100.0% 147.9 / NA 35coding (794/1413 nt)pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/16);  total (19/16)

AAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGA  >  NC_000913/1756356‑1756617
                                                                                                                                       |                                                                                                                              
aaaTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaa                                                                                                                              >  1:51072/1‑138 (MQ=255)
aaaTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaa                                                                                                                              <  2:51072/138‑1 (MQ=255)
    cGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaaatgta                                                                                                                         >  1:1093231/1‑135 (MQ=255)
    cGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaaatgta                                                                                                                         >  1:889574/1‑135 (MQ=255)
                  gCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTaaa                                                                                                          <  2:923347/139‑1 (MQ=255)
                    cTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGt                                                                                                        <  2:323211/139‑1 (MQ=255)
                            cTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCa                                                                                                >  1:368037/1‑139 (MQ=255)
                            cTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCa                                                                                                >  2:524239/1‑139 (MQ=255)
                             ttCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCAc                                                                                               >  1:909116/1‑139 (MQ=255)
                               cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                                             <  2:368037/139‑1 (MQ=255)
                               cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                                             <  2:117217/139‑1 (MQ=255)
                                           cGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGc                                                                                 <  1:761066/139‑1 (MQ=255)
                                                          catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCa                                                                  >  2:805680/1‑139 (MQ=255)
                                                            tcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaaa                                                                >  2:714582/1‑139 (MQ=255)
                                                                ggTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAcc                                                            >  1:168202/1‑139 (MQ=255)
                                                                ggTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAcc                                                            <  2:193658/139‑1 (MQ=255)
                                                                     cgcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGc                                                       >  1:79894/1‑139 (MQ=255)
                                                                                    gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTg                                                                                                                  <  1:537609/65‑1 (MQ=255)
                                                                                    gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTg                                                                                                                  >  2:537609/1‑65 (MQ=255)
                                                                                      gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGc                                      <  2:1093231/139‑1 (MQ=255)
                                                                                         gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                   <  2:339577/139‑1 (MQ=255)
                                                                                             tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                               >  1:364160/1‑139 (MQ=255)
                                                                                             tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                               >  2:648790/1‑139 (MQ=255)
                                                                                               ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCGCTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                             >  1:636795/1‑139 (MQ=255)
                                                                                                 ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                                             >  1:350490/1‑73 (MQ=255)
                                                                                                 ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                                             <  2:350490/73‑1 (MQ=255)
                                                                                                 ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                           >  2:1020096/1‑139 (MQ=255)
                                                                                                  gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                          >  2:329803/1‑139 (MQ=255)
                                                                                                        gaagTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTAt                                                                                                  >  1:565465/1‑61 (MQ=255)
                                                                                                        gaagTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTAt                                                                                                  <  2:565465/61‑1 (MQ=255)
                                                                                                         aagTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGcc                   <  2:528491/139‑1 (MQ=255)
                                                                                                                  tCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc          <  2:990997/139‑1 (MQ=255)
                                                                                                                     cccAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt       >  2:534451/1‑139 (MQ=255)
                                                                                                                     cccAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt       >  1:580962/1‑139 (MQ=255)
                                                                                                                       cAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc     <  2:909116/139‑1 (MQ=255)
                                                                                                                       cAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTAc     <  1:302804/139‑1 (MQ=255)
                                                                                                                         gaagaTGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTg   >  1:665183/1‑139 (MQ=255)
                                                                                                                          aagaTGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGa  <  2:636795/139‑1 (MQ=255)
                                                                                                                          aagaTGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGAGACGTTGCAAACGCCATCCTCGACGGTACTGa  >  2:828580/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                              
AAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGA  >  NC_000913/1756356‑1756617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A