Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	70	59.9	2639336	94.5%	2494172	113.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,736,687	T→C	V181A (GTG→GCG)	bamD →	BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,736,687	0	T	C	100.0%	162.6 / NA	44	V181A (GTG→GCG)	bamD	BamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/25); total (19/25)

TCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGC  >  NC_000913/2736553‑2736821

tcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  2:1061446/139‑1 (MQ=255)

tcCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  2:978238/139‑1 (MQ=255)

  cTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  <  1:630079/139‑1 (MQ=255)

   tCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAg                                                                                                                                 <  1:304736/139‑1 (MQ=255)

   tCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAg                                                                                                                                 <  1:791999/139‑1 (MQ=255)

                    cGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTgg                                                                                                                >  2:167195/1‑139 (MQ=255)

                              cTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  <  2:917926/111‑1 (MQ=255)

                              cTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  >  1:917926/1‑111 (MQ=255)

                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  1:1221748/108‑1 (MQ=255)

                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    >  2:1221748/1‑108 (MQ=255)

                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  1:124372/139‑1 (MQ=255)

                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  2:723306/139‑1 (MQ=255)

                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  2:1086120/139‑1 (MQ=255)

                               ttttCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTg                                                                                                     <  1:167195/139‑1 (MQ=255)

                                           gTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAAcc                                                                                         >  1:903789/1‑139 (MQ=255)

                                            tGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACcg                                                                                        >  1:511655/1‑139 (MQ=255)

                                               gcgGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATACGAATACTCCGCGGcc                                                                                                                                    >  1:939568/1‑92 (MQ=255)

                                               gcgGCTATCCGAACAGTCAGTACACCACAGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  2:939568/92‑1 (MQ=255)

                                                   cTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGaa                                                                                 >  1:254777/1‑139 (MQ=255)

                                                               tcagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTgcgc                                                                     <  2:903789/139‑1 (MQ=255)

                                                                cagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTa                                                                                                                               <  1:735779/80‑1 (MQ=255)

                                                                cagtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTa                                                                                                                               >  2:735779/1‑80 (MQ=255)

                                                                 agtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    <  1:55699/74‑1 (MQ=255)

                                                                 agtACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGcc                                                                                                                                    >  2:55699/1‑74 (MQ=255)

                                                                   tACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACt                                                                 <  2:47680/139‑1 (MQ=255)

                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCgg                                                                                                                                      <  2:300176/65‑1 (MQ=255)

                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCgg                                                                                                                                      >  1:300176/1‑65 (MQ=255)

                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCg                                                            <  2:681322/139‑1 (MQ=255)

                                                                        caccGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCg                                                            <  2:254777/139‑1 (MQ=255)

                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  <  2:1141357/64‑1 (MQ=255)

                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGa                                                                                                                                  >  1:1141357/1‑64 (MQ=255)

                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGt                                                                                                                  >  2:1166756/1‑80 (MQ=255)

                                                                             aTGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGt                                                                                                                  <  1:1166756/80‑1 (MQ=255)

                                                                                           cTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAATGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTg                                         >  1:857814/1‑139 (MQ=255)

                                                                                            tGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGa                                        >  1:610927/1‑139 (MQ=255)

                                                                                                      gAAAGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCg                              <  2:511655/139‑1 (MQ=255)

                                                                                                         aGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAcc                                                      <  2:18385/112‑1 (MQ=255)

                                                                                                         aGATCGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATAcc                                                      >  1:18385/1‑112 (MQ=255)

                                                                                                             cGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGa                                                          <  2:876231/104‑1 (MQ=255)

                                                                                                             cGTCTGGCGAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGa                                                          >  1:876231/1‑104 (MQ=255)

                                                                                                                     gAAATATGAATACTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCa               <  2:610927/139‑1 (MQ=255)

                                                                                                                                 cTCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatg   >  1:658231/1‑139 (MQ=255)

                                                                                                                                  tCCGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTcagatgc  >  2:25112/1‑139 (MQ=255)

                                                                                                                                   ccGCGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCAt              >  1:1280695/1‑126 (MQ=255)

TCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACCACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTGGCCGAGTACTATACAGAACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGC  >  NC_000913/2736553‑2736821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A