Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 70 59.9 2639336 94.5% 2494172 113.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,516,213 G→A A365V (GCG→GTG)  damX ← cell division protein that binds to the septal ring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,516,2130GA98.0% 167.9 / ‑5.2 49A365V (GCG→GTG) damXcell division protein that binds to the septal ring
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/24);  minor base C (0/1);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCAGCAGCGGGTGTTGCTGTGGT  >  NC_000913/3516078‑3516340
                                                                                                                                       |                                                                                                                               
aCATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCAccc                                                                                                                              <  1:59683/139‑1 (MQ=255)
aCATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCAccc                                                                                                                              <  1:1163250/139‑1 (MQ=255)
 cATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCa                                                                                                                             <  1:551869/139‑1 (MQ=255)
         ggCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCa                                                                                                                     >  2:1031646/1‑139 (MQ=255)
           cAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAgg                                                                                                                   <  1:282073/139‑1 (MQ=255)
           cAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAgg                                                                                                                   <  1:711100/139‑1 (MQ=255)
              tGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTg                                                                                                                >  2:1289977/1‑139 (MQ=255)
               gTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGt                                                                                                               <  2:629704/139‑1 (MQ=255)
                        gCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACAACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTag                                                                                                      >  1:485392/1‑139 (MQ=255)
                         cTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTaga                                                                                                     >  2:279494/1‑139 (MQ=255)
                         cTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTaga                                                                                                     >  1:268731/1‑139 (MQ=255)
                         cTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTaga                                                                                                     >  2:564665/1‑139 (MQ=255)
                         cTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGGTaga                                                                                                     >  1:259647/1‑139 (MQ=255)
                                  ccTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCt                                                                                            <  1:387503/139‑1 (MQ=255)
                                                gCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAgg                                                                                                                   <  1:959368/102‑1 (MQ=255)
                                                gCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAgg                                                                                                                   >  2:959368/1‑102 (MQ=255)
                                                gCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAgtgt                                                                              >  1:320886/1‑139 (MQ=255)
                                                gCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAgtgt                                                                              >  2:917977/1‑139 (MQ=255)
                                                          cAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGa                                                                    <  2:268731/139‑1 (MQ=255)
                                                            gAAGCCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATCGACAACGTAGTTTTTCAGATTCTCTTTCTTCCCCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACg                                                                  <  1:856002/139‑1 (MQ=255)
                                                               aCCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTg                                                               <  1:1289977/139‑1 (MQ=255)
                                                                 cAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGcc                                                             <  2:320886/139‑1 (MQ=255)
                                                                        tACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCa                                                      <  1:917977/139‑1 (MQ=255)
                                                                         aCCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCaa                                                     >  1:821353/1‑139 (MQ=255)
                                                                             cGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGaa                                                 <  2:485392/139‑1 (MQ=255)
                                                                              ggCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGaa                                                 <  1:140216/138‑1 (MQ=255)
                                                                              ggCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGaa                                                 >  2:140216/1‑138 (MQ=255)
                                                                               gCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAAcc                                               <  1:20155/139‑1 (MQ=255)
                                                                                   aCCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCAccc                                                                                                                              >  1:654487/1‑56 (MQ=255)
                                                                                   aCCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCAccc                                                                                                                              <  2:654487/56‑1 (MQ=255)
                                                                                   aCCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAGCGAACCAACa                                           >  1:50803/1‑139 (MQ=255)
                                                                                   aCCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACa                                           >  2:679586/1‑139 (MQ=255)
                                                                                               cgtTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTc                               >  1:347067/1‑139 (MQ=255)
                                                                                                  ttCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTc                            >  2:523797/1‑139 (MQ=255)
                                                                                                         aCAACGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCcag                     >  1:866586/1‑139 (MQ=255)
                                                                                                             cGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGt                                                                                                               <  1:2985/45‑1 (MQ=255)
                                                                                                             cGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGt                                                                                                               >  2:2985/1‑45 (MQ=255)
                                                                                                             cGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAAc                                                <  2:249367/108‑1 (MQ=255)
                                                                                                             cGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAAc                                                >  1:249367/1‑108 (MQ=255)
                                                                                                             cGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCagcagc                 <  1:1031646/139‑1 (MQ=255)
                                                                                                             cGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCagcagc                 >  1:584433/1‑139 (MQ=255)
                                                                                                             cGTAGTTTTTCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCagcagc                 >  1:631090/1‑139 (MQ=255)
                                                                                                                  tttttCAGATTCTCTTTCTTCACCCTACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGGACCAACAGTACCTGCGGTCTTCGCCCCAGCAGCGGgtg            <  1:1159194/139‑1 (MQ=255)
                                                                                                                  tttttCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCAGCAGCGGgtg            <  2:671439/139‑1 (MQ=255)
                                                                                                                  tttttCAGATTCTCTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCAGCAGCGGgtg            <  1:679586/139‑1 (MQ=255)
                                                                                                                            tctcTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAgg                                                                                                   >  1:942305/1‑42 (MQ=255)
                                                                                                                            tctcTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAgg                                                                                                   <  2:942305/42‑1 (MQ=255)
                                                                                                                            tctcTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAg                                                                                         >  2:856083/1‑52 (MQ=255)
                                                                                                                            tctcTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAg                                                                                         <  1:856083/52‑1 (MQ=255)
                                                                                                                            tctcTTTCTTCACCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCAGCAGCGGGTGTTGCTGTGGt  <  1:279494/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                               
ACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAGAGGAACTGCTCAGCTGCAGAGTGTAATGGCTGGACGGTGCCGATTTCAACGAACCAACATTACCTGCGGTCTTCGCCCCAGCAGCGGGTGTTGCTGTGGT  >  NC_000913/3516078‑3516340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A