Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A7 F1 I2 R1 | 94 | 59.5 | 3027142 | 91.9% | 2781943 | 108.1 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,907,539 | T→C | Y18C (TAC→TGC) | yobF ← | DUF2527 family heat‑induced protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,907,539 | 0 | T | C | 100.0% | 278.3 / NA | 76 | Y18C (TAC→TGC) | yobF | DUF2527 family heat‑induced protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (40/36); total (40/36) | |||||||||||
TTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATG > NC_000913/1907410‑1907669 | ttAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATtcaacgt > 2:302249/1‑139 (MQ=255) aCCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATtcaacgtcaa < 1:599853/139‑1 (MQ=255) ccTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGtt > 1:85275/1‑139 (MQ=255) aTCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCa > 1:1174809/1‑139 (MQ=255) tGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGAct > 1:237857/1‑139 (MQ=255) tGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGAct > 1:1508539/1‑139 (MQ=255) aaaaTTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGa < 1:302249/139‑1 (MQ=255) ttcGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGaa < 1:1047829/139‑1 (MQ=255) ccGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTa > 1:188595/1‑139 (MQ=255) tAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCg < 2:1174809/139‑1 (MQ=255) gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGc > 1:65949/1‑103 (MQ=255) gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGc < 2:65949/103‑1 (MQ=255) gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacac > 2:1276711/1‑108 (MQ=255) gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacac < 1:1276711/108‑1 (MQ=255) gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa > 1:613499/1‑113 (MQ=255) gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa < 2:613499/113‑1 (MQ=255) aTAAAACTGACACGTTACTGCATAAGGCACCAATATAAGGCTCGGCAGAGAAGCTGCATTCAACGACAACGTGTTTACTTAGGACTTCTTTACTGAAAACGCCACACATAAACAGAACTGTACCTCGTTTAACCCGaaa < 2:994722/139‑1 (MQ=255) aTAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGaa < 2:142217/117‑1 (MQ=255) aTAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGaa > 1:142217/1‑117 (MQ=255) aaCTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTg < 2:188595/139‑1 (MQ=255) aCTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTg > 1:938063/1‑138 (MQ=255) aCTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTg < 2:938063/138‑1 (MQ=255) acacATTACTGCATGAGGCACCAATATAAGGCTCGGGAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCAACACATAAACAGAACTGTACGTCGTTTAACGCGAAATCTGTTATc > 2:1409264/1‑139 (MQ=255) acacATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATc > 1:252874/1‑139 (MQ=255) caTTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATcaca < 2:85275/139‑1 (MQ=255) tACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATAc > 2:1458770/1‑139 (MQ=255) aCTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGttt > 1:272484/1‑114 (MQ=255) aCTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGttt < 2:272484/114‑1 (MQ=255) gCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACg < 1:1336264/139‑1 (MQ=255) cATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACt > 1:1411979/1‑59 (MQ=255) cATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACt < 2:1411979/59‑1 (MQ=255) tGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGc < 2:325175/80‑1 (MQ=255) tGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGc > 1:325175/1‑80 (MQ=255) gAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATtcaacgtcaa < 1:196830/46‑1 (MQ=255) gAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATtcaacgtcaa > 2:196830/1‑46 (MQ=255) gAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTaa > 1:238684/1‑139 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaaaa < 2:1425496/74‑1 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaaaa > 1:1425496/1‑74 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaa < 2:1403630/72‑1 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaa > 1:1403630/1‑72 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACAt > 1:1288168/1‑84 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACAt < 2:1288168/84‑1 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa > 2:460151/1‑128 (MQ=255) ggCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa < 1:460151/128‑1 (MQ=255) cAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacac > 2:687949/1‑77 (MQ=255) cAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacac < 1:687949/77‑1 (MQ=255) cAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa > 1:564124/1‑82 (MQ=255) cAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa < 2:564124/82‑1 (MQ=255) ataAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc < 1:1458770/139‑1 (MQ=255) ataAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc < 2:1508539/139‑1 (MQ=255) taAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGcc > 2:278645/1‑139 (MQ=255) ggCTCGGCAGAGAAGCGGCATTCAAAGCCAACGTGTTTACCCAGGACTTCTTTACTGAAAATGCCCCACATAAAAAGAACTGTACTTCGATTAACCCGAAAACTGTTATAACATACAACATTAATTATGGCAGGCAAtt > 2:741563/1‑139 (MQ=255) cTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacac < 2:1184032/67‑1 (MQ=255) cTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacac > 1:1184032/1‑67 (MQ=255) cTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATcaca < 2:1149865/111‑1 (MQ=255) cTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATcaca > 1:1149865/1‑111 (MQ=255) cTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAtttt > 2:559903/1‑139 (MQ=255) cGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGtt > 1:1102473/1‑103 (MQ=255) cGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGtt < 2:1102473/103‑1 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGaa < 1:133349/74‑1 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGaa > 2:133349/1‑74 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGt > 2:1503965/1‑85 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGt < 1:1503965/85‑1 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTg > 1:556387/1‑100 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTg < 2:556387/100‑1 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTAt > 1:466238/1‑104 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTAt < 2:466238/104‑1 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACg > 2:1404527/1‑115 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACg < 1:1404527/115‑1 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGcc < 2:1347059/131‑1 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGcc > 1:1347059/1‑131 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa > 1:216063/1‑139 (MQ=255) ggCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa > 1:1446622/1‑139 (MQ=255) agaAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa < 2:1064046/105‑1 (MQ=255) agaAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa > 1:1064046/1‑105 (MQ=255) agaAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATg < 2:216063/139‑1 (MQ=255) | TTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATG > NC_000913/1907410‑1907669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |