Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
20.9 |
912876 |
97.7% |
891879 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,907,616 |
C→T |
intergenic (‑25/+645) |
yobF ← / ← yebO |
DUF2527 family heat‑induced protein/putative inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,907,616 | 0 | C | T | 100.0%
| 135.2
/ NA
| 40 | intergenic (‑25/+645) | yobF/yebO | DUF2527 family heat‑induced protein/putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/18); total (22/18) |
ACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCA > NC_000913/1907488‑1907749
|
aCTGACACATTACTGCATGAGGCACCAATCTAAGGCTCGGCAGAGCAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGt > 2:263776/1‑139 (MQ=255)
gACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTAt < 1:318824/139‑1 (MQ=255)
cATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGt > 1:245068/1‑139 (MQ=255)
tGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTACTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTATACCTCGTTTAACTCGACATCTCTTATCATATACAAcaata > 2:410203/1‑135 (MQ=255)
ggCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt > 2:39118/1‑139 (MQ=255)
ggCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt > 1:456269/1‑139 (MQ=255)
cAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACg > 1:192772/1‑128 (MQ=255)
cAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACg < 2:192772/128‑1 (MQ=255)
cTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAtttt > 2:445700/1‑139 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTg < 2:356773/100‑1 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTg > 1:356773/1‑100 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTAt < 2:277899/104‑1 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTAt > 1:277899/1‑104 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATa < 2:98695/110‑1 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATa > 1:98695/1‑110 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt > 1:163821/1‑121 (MQ=255)
ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt < 2:163821/121‑1 (MQ=255)
gCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaaa < 2:456269/139‑1 (MQ=255)
cAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAAc < 1:263776/139‑1 (MQ=255)
acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATc < 2:362029/80‑1 (MQ=255)
acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATc > 1:362029/1‑80 (MQ=255)
acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAAt > 1:60904/1‑139 (MQ=255)
acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAAt > 1:345018/1‑139 (MQ=255)
tttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGa > 2:140525/1‑139 (MQ=255)
cAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTc > 1:326535/1‑139 (MQ=255)
ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCtt > 1:166514/1‑139 (MQ=255)
tttACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACttt > 2:393792/1‑139 (MQ=255)
aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGca < 1:39118/139‑1 (MQ=255)
gCCACACATAAACAGAACTGTACCTCGTTTAACTCGaaa < 1:117511/39‑1 (MQ=255)
cccACACATAAACAGAACTGTACCTCGTTTAACTCGaaa > 2:117511/2‑39 (MQ=255)
aTAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAAc < 2:245068/139‑1 (MQ=255)
aCAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGaa < 1:393792/139‑1 (MQ=255)
tGTACCTCTTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTGGCACACTTATGCAACGGAATACgcgc < 1:368678/139‑1 (MQ=255)
tGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACgcgc < 1:140525/139‑1 (MQ=255)
tACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCg > 1:329019/1‑139 (MQ=255)
tACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCg > 1:405977/1‑139 (MQ=255)
cGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATAcgcg > 1:151178/1‑131 (MQ=255)
cGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATAcgcg < 2:151178/131‑1 (MQ=255)
ttAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCa < 2:198943/139‑1 (MQ=255)
aaCTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACgcgc < 1:375869/127‑1 (MQ=255)
|
ACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCA > NC_000913/1907488‑1907749
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
