Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
94 |
59.5 |
3027142 |
91.9% |
2781943 |
108.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,263,580 |
A→G |
C27C (TGT→TGC) |
tdcD ← |
propionate kinase/acetate kinase C, anaerobic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,263,580 | 0 | A | G | 100.0%
| 256.9
/ NA
| 69 | C27C (TGT→TGC) | tdcD | propionate kinase/acetate kinase C, anaerobic |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (36/33); total (36/33) |
GCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGA > NC_000913/3263464‑3263705
|
gCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACgg > 1:383747/1‑139 (MQ=255)
ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGa > 2:396144/1‑139 (MQ=255)
ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGaaa > 2:814245/1‑139 (MQ=255)
tAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg < 2:315066/139‑1 (MQ=255)
cTGTGGTGAGCCAGCGGTGCTGGCTCTCCTCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc > 1:1319697/1‑139 (MQ=255)
tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac < 1:1247773/139‑1 (MQ=255)
tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac > 1:938898/1‑139 (MQ=255)
ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcaca > 1:1421713/1‑139 (MQ=255)
gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCGGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGTACcac > 1:966125/1‑135 (MQ=255)
gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac < 2:966125/135‑1 (MQ=255)
aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcgca < 1:1040183/90‑1 (MQ=255)
aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATGAATACTTcgca > 2:1040183/1‑90 (MQ=255)
gCCAGCGGTGCTGGCTCTCCTCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaa < 2:1319697/139‑1 (MQ=255)
ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCt > 2:649980/1‑94 (MQ=255)
ccAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCt < 1:649980/94‑1 (MQ=255)
gCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaaa < 2:1421713/139‑1 (MQ=255)
cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta > 2:684686/1‑120 (MQ=255)
cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta < 1:684686/120‑1 (MQ=255)
cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaa < 2:383747/139‑1 (MQ=255)
ccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGACAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACTACAGTTAATAACCAAAAAAACCGGaaa > 2:1066728/1‑139 (MQ=255)
ttACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattcatt < 2:938898/139‑1 (MQ=255)
aCGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAAttcattca > 2:113772/1‑139 (MQ=255)
aCGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAAttcattca > 2:599594/1‑139 (MQ=255)
aCGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAAttcattca < 2:1393394/139‑1 (MQ=255)
aCGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAAttcattca > 2:3161/1‑139 (MQ=255)
ggATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACTGGAAACTCATTCAtc > 2:365188/1‑139 (MQ=255)
ggATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg < 1:696016/125‑1 (MQ=255)
ggATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg > 2:696016/1‑125 (MQ=255)
aTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca < 1:104170/101‑1 (MQ=255)
aTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca > 2:104170/1‑101 (MQ=255)
tAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCAt > 1:986149/1‑68 (MQ=255)
tAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCAt < 2:986149/68‑1 (MQ=255)
aGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtctc < 2:999619/136‑1 (MQ=255)
aGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtctc > 1:999619/1‑136 (MQ=255)
tttCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCt > 1:1430209/1‑139 (MQ=255)
tCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGa < 1:129951/65‑1 (MQ=255)
tCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGa > 2:129951/1‑65 (MQ=255)
tACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGaa > 2:652993/1‑74 (MQ=255)
tACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGaa < 1:652993/74‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAg < 1:588099/44‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAg > 2:588099/1‑44 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACg > 2:81002/1‑48 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACg < 1:81002/48‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATc < 2:1031252/60‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATc > 1:1031252/1‑60 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc < 1:336373/70‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc > 2:336373/1‑70 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca < 1:170388/72‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca > 2:170388/1‑72 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCTGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc < 1:365188/139‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAatt > 1:275699/1‑100 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAatt < 2:275699/100‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtctc > 1:540164/1‑110 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtctc < 2:540164/110‑1 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATGCTGAGTTACGGATTAAAAc > 2:986113/1‑139 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc > 1:97168/1‑139 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc > 1:607972/1‑139 (MQ=255)
tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc > 1:160767/1‑139 (MQ=255)
ggCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAg < 1:814245/139‑1 (MQ=255)
gCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGt < 2:1109431/139‑1 (MQ=255)
tGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATAc < 2:1430209/139‑1 (MQ=255)
gACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACg < 1:113772/139‑1 (MQ=255)
aCATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCtttt > 1:435991/1‑101 (MQ=255)
aCATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCtttt < 2:435991/101‑1 (MQ=255)
aCATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGa < 1:3161/139‑1 (MQ=255)
tAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg < 1:490910/50‑1 (MQ=255)
tAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg > 2:490910/1‑50 (MQ=255)
aaTACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg > 2:1440590/1‑49 (MQ=255)
aaTACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg < 1:1440590/49‑1 (MQ=255)
aTACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTtc > 1:454395/1‑96 (MQ=255)
aTACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTtc < 2:454395/96‑1 (MQ=255)
|
GCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGA > NC_000913/3263464‑3263705
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
