Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	65	25.3	1027584	98.0%	1007032	120.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,600	T→C	*396Q (TAG→CAG)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,600	0	T	C	100.0%	130.4 / NA	34	*396Q (TAG→CAG)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/16); total (18/16)

CTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCT  >  NC_000913/3269465‑3269725

cTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGa                                                                                                                            >  1:400267/1‑139 (MQ=255)

  cGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACt                                                                                                                          >  2:736/1‑139 (MQ=255)

                   ctcCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACaaa                                                                                                         <  2:191934/139‑1 (MQ=255)

                     ccGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGt                                                                                                       <  1:404445/139‑1 (MQ=255)

                       ggACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCt                                                                                                     <  2:88531/139‑1 (MQ=255)

                        gACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCta                                                                                                    <  2:131071/139‑1 (MQ=255)

                                      ggTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTa                                                                                      <  1:305127/139‑1 (MQ=255)

                                               atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:388855/139‑1 (MQ=255)

                                               atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:327842/139‑1 (MQ=255)

                                                         tatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                   <  1:426212/139‑1 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     >  1:504294/1‑136 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     <  1:297746/136‑1 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     <  2:504294/136‑1 (MQ=255)

                                                          atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     >  2:297746/1‑136 (MQ=255)

                                                               gCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGc                                                             >  2:29944/1‑139 (MQ=255)

                                                                    taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:52176/118‑1 (MQ=255)

                                                                    taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             >  1:52176/1‑118 (MQ=255)

                                                                       cTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAg                                                     >  2:169005/1‑139 (MQ=255)

                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACaaa                                                                                                         >  1:22214/1‑82 (MQ=255)

                                                                            agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACaaa                                                                                                         <  2:22214/82‑1 (MQ=255)

                                                                               aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                   <  1:31879/117‑1 (MQ=255)

                                                                               aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                   >  2:31879/1‑117 (MQ=255)

                                                                                 cAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCa                                           >  2:87228/1‑139 (MQ=255)

                                                                                          aCCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCgg                                  <  1:29944/139‑1 (MQ=255)

                                                                                                           gTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGAcgcg                                   >  2:485270/1‑121 (MQ=255)

                                                                                                           gTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGAcgcg                                   <  1:485270/121‑1 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAc               >  2:136199/1‑139 (MQ=255)

                                                                                                             aTGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAc               >  2:441202/1‑139 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:219301/74‑1 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             >  1:219301/1‑74 (MQ=255)

                                                                                                                aTTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGc            >  1:397689/1‑139 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaa          >  1:358629/1‑139 (MQ=255)

                                                                                                                  tACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaa          >  1:268955/1‑139 (MQ=255)

                                                                                                                          tttAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCt  >  1:178794/1‑139 (MQ=255)

CTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCT  >  NC_000913/3269465‑3269725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A