Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	94	59.5	3027142	91.9%	2781943	108.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	95.4%	299.4 / 5.8	87	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (39/44); total (41/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTT  >  NC_000913/3379121‑3379382

gTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGt‑tt                                                                                                                               <  1:818742/139‑1 (MQ=255)

gTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGt‑tt                                                                                                                               <  2:305174/139‑1 (MQ=255)

                gTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcgc                                                                                                              <  2:353878/139‑1 (MQ=255)

                    tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGACGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAAc                                                                                                          >  2:367101/1‑139 (MQ=255)

                     gCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAAcc                                                                                                         <  1:346129/139‑1 (MQ=255)

                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcgc                                                                                                              >  2:1398718/1‑133 (MQ=255)

                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcgc                                                                                                              <  1:1398718/133‑1 (MQ=255)

                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCa                                                                                                        >  1:1089885/1‑139 (MQ=255)

                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCa                                                                                                        >  2:1513075/1‑139 (MQ=255)

                          cTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGt                                                                                                    <  1:972930/139‑1 (MQ=255)

                                   tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CCCCCGCCAGCGCCAAcc                                                                                                         >  1:68330/1‑124 (MQ=255)

                                   tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CCCCCGCCAGCGCCAAcc                                                                                                         <  2:68330/124‑1 (MQ=255)

                                     gACGCACAGCGTAGTAAAAGAGACAGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                                                         >  2:282448/1‑139 (MQ=255)

                                        gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGG‑TGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                                                     <  2:924046/139‑1 (MQ=255)

                                         cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                                                     <  2:1089885/139‑1 (MQ=255)

                                              cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                                                >  2:632284/1‑139 (MQ=255)

                                                  gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                                                            <  1:632284/139‑1 (MQ=255)

                                                      aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                                                        >  2:310878/1‑139 (MQ=255)

                                                        gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                                                      >  2:104048/1‑139 (MQ=255)

                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTCTC‑CC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                                                   >  1:1007627/1‑139 (MQ=255)

                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      <  2:1053954/120‑1 (MQ=255)

                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      >  1:1053954/1‑120 (MQ=255)

                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                                                   >  2:1373035/1‑139 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:645824/1‑139 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:791252/1‑139 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:1405225/1‑139 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  1:215427/1‑139 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:1088742/1‑139 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  1:1321361/1‑139 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑ccc                                                                                                                        <  2:405276/83‑1 (MQ=255)

                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑cc‑ccc                                                                                                                        >  1:405276/1‑83 (MQ=255)

                                                             cGCCAGCGTCTGGCTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcg                                                                                                               >  2:138473/1‑93 (MQ=255)

                                                             cGCCAGCGTCTGGCTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcg                                                                                                               <  1:138473/93‑1 (MQ=255)

                                                               ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               <  1:791252/139‑1 (MQ=255)

                                                               ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               <  2:400993/139‑1 (MQ=255)

                                                                cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                                              <  1:367101/139‑1 (MQ=255)

                                                                cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                                              <  1:1088742/139‑1 (MQ=255)

                                                                 aGCGTCTGTTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CCTCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                                             <  1:896431/139‑1 (MQ=255)

                                                                 aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                                             <  1:104048/139‑1 (MQ=255)

                                                                            ctcGACGCCCATTTTTGCTAATGGCCGATGGTTGAGTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                                                  <  2:722200/139‑1 (MQ=255)

                                                                             tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                                                 <  2:522142/139‑1 (MQ=255)

                                                                                aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                                              >  2:1445099/1‑139 (MQ=255)

                                                                                    ccAGTGTTGCTAATGGCCGATGGTTAATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag                                          <  1:973869/139‑1 (MQ=255)

                                                                                     cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt                                         <  1:272919/139‑1 (MQ=255)

                                                                                        tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag                                      <  1:1445099/139‑1 (MQ=255)

                                                                                             cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCa                                                                                                        <  2:1027692/68‑1 (MQ=255)

                                                                                             cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCa                                                                                                        >  1:1027692/1‑68 (MQ=255)

                                                                                              tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcgc                                                                                                              >  1:1173724/1‑61 (MQ=255)

                                                                                              tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcgc                                                                                                              <  2:1173724/61‑1 (MQ=255)

                                                                                                 tGGCCGATGGTTGATTTATAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAGCCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTCGCGAAAGCCACAGATCCGCCTGAGTGAGTGTACGCAg                             >  2:1035745/1‑139 (MQ=255)

                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      <  1:169224/81‑1 (MQ=255)

                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      >  2:169224/1‑81 (MQ=255)

                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               >  1:689519/1‑120 (MQ=255)

                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               <  2:689519/120‑1 (MQ=255)

                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag                                          <  2:689943/125‑1 (MQ=255)

                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag                                          >  1:689943/1‑125 (MQ=255)

                                                                                                    ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACg                          <  1:1373035/139‑1 (MQ=255)

                                                                                                     cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      <  1:56432/78‑1 (MQ=255)

                                                                                                     cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      >  2:56432/1‑78 (MQ=255)

                                                                                                       aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAAcc                                                                                                         <  2:20960/57‑1 (MQ=255)

                                                                                                       aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAAcc                                                                                                         >  1:20960/1‑57 (MQ=255)

                                                                                                          gTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt                                         <  2:1486714/118‑1 (MQ=255)

                                                                                                          gTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt                                         >  1:1486714/1‑118 (MQ=255)

                                                                                                          gTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATc                    <  2:907174/139‑1 (MQ=255)

                                                                                                           ttGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               >  2:1189442/1‑111 (MQ=255)

                                                                                                           ttGATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               <  1:1189442/111‑1 (MQ=255)

                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcgc                                                                                                              >  1:1141941/1‑46 (MQ=255)

                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAgcgc                                                                                                              <  2:1141941/46‑1 (MQ=255)

                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGcc                                                                                                             <  1:258239/47‑1 (MQ=39)

                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGcc                                                                                                             >  2:258239/1‑47 (MQ=39)

                                                                                                                  cTAACGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAg            <  1:380056/139‑1 (MQ=255)

                                                                                                                     aCGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTAt                                                                                                >  1:1212974/1‑52 (MQ=255)

                                                                                                                     aCGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTAt                                                                                                <  2:1212974/52‑1 (MQ=255)

                                                                                                                     aCGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                                               >  2:1097633/1‑69 (MQ=255)

                                                                                                                     aCGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                                               <  1:1097633/69‑1 (MQ=255)

                                                                                                                     aCGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                                                   >  2:1460457/1‑97 (MQ=255)

                                                                                                                     aCGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                                                   <  1:1460457/97‑1 (MQ=255)

                                                                                                                      cGTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTc        <  1:1465345/139‑1 (MQ=255)

                                                                                                                       gTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc       <  2:215427/139‑1 (MQ=255)

                                                                                                                       gTCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc       <  2:1321361/139‑1 (MQ=255)

                                                                                                                        tCGGTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCa      >  2:1016567/1‑139 (MQ=255)

                                                                                                                          ggTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               <  1:119093/80‑1 (MQ=255)

                                                                                                                          ggTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               >  2:119093/1‑80 (MQ=255)

                                                                                                                          ggTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                                                         >  2:231958/1‑86 (MQ=255)

                                                                                                                          ggTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                                                         <  1:231958/86‑1 (MQ=255)

                                                                                                                          ggTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg    >  1:1404508/1‑139 (MQ=255)

                                                                                                                          ggTGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg    >  1:199443/1‑139 (MQ=255)

                                                                                                                            tGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  <  2:199443/139‑1 (MQ=255)

                                                                                                                            tGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  <  1:282448/139‑1 (MQ=255)

                                                                                                                            tGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  <  2:137540/139‑1 (MQ=255)

                                                                                                                            tGAATTTTCTCGT‑TTCCC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  >  1:571431/1‑139 (MQ=255)

GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGT‑TT‑CC‑CCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTT  >  NC_000913/3379121‑3379382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A