Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	77	35.1	1569012	96.6%	1515665	112.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,649	A→G	L422P (CTG→CCG)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,649	0	A	G	100.0%	86.7 / NA	25	L422P (CTG→CCG)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/10); total (15/10)

GTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACAGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCAACATCC  >  NC_000913/1629520‑1629784

gTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCAc                                                                                                                                >  2:605103/1‑139 (MQ=255)

                    acTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTccc                                                                                                            <  1:605103/139‑1 (MQ=255)

                         tGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTtc                                                                                                       >  1:259041/1‑139 (MQ=255)

                         tGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTtc                                                                                                       <  1:623692/139‑1 (MQ=255)

                               gCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAac                                                                                                 <  1:234308/139‑1 (MQ=255)

                                       tcaCCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTTCCTGTTCATCAACACCACCGa                                                                                         <  1:619040/139‑1 (MQ=255)

                                                                          gACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAgcgc                                                          >  2:780091/1‑135 (MQ=255)

                                                                          gACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAgcgc                                                          <  1:780091/135‑1 (MQ=255)

                                                                                                      tGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTa                                                                                                                       <  2:489989/46‑1 (MQ=255)

                                                                                                      tGTACAGCCTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTa                                                                                                                       >  1:489989/1‑46 (MQ=255)

                                                                                                             ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTccc                                                                                                            >  2:266283/1‑50 (MQ=255)

                                                                                                             ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTccc                                                                                                            <  1:266283/50‑1 (MQ=255)

                                                                                                             ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCg                   >  1:468912/1‑139 (MQ=255)

                                                                                                             ccTTCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCg                   >  2:386713/1‑139 (MQ=255)

                                                                                                                tCTGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAAc                >  2:738145/1‑139 (MQ=255)

                                                                                                                  tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGccc                                                               <  2:142394/90‑1 (MQ=255)

                                                                                                                  tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGccc                                                               >  1:142394/1‑90 (MQ=255)

                                                                                                                  tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAg              >  1:292611/1‑139 (MQ=255)

                                                                                                                  tGAATAACAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAg              >  1:401967/1‑139 (MQ=255)

                                                                                                                         cAGGTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATACAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCaa       >  2:630976/1‑139 (MQ=255)

                                                                                                                            gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCaa                                                                                                  >  1:299599/1‑45 (MQ=255)

                                                                                                                            gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCaa                                                                                                  <  2:299599/45‑1 (MQ=255)

                                                                                                                            gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATgcg                                <  2:450895/111‑1 (MQ=255)

                                                                                                                            gTAACGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATgcg                                >  1:450895/1‑111 (MQ=255)

                                                                                                                              aaCGGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCAACATcc  >  2:721834/1‑139 (MQ=255)

GTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTACTTTGTACAGCCTTCTGAATAACAGGTAACAGTGGGTCACTGATTTCTATCGGATTTCCCTGTTCATCAACACCACCGACATAACGCATCCAACCCGCGACGCCCAGCGCCAGCAGATCGAACTTGCTGTCATGCGCCAGATGCCAGCGAACAGAATCCAACATCC  >  NC_000913/1629520‑1629784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A