Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
77 |
35.1 |
1569012 |
96.6% |
1515665 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,189,791 |
A→G |
Y374H (TAT→CAT) |
yehB ← |
putative outer membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,189,791 | 0 | A | G | 100.0%
| 93.8
/ NA
| 27 | Y374H (TAT→CAT) | yehB | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/12); total (15/12) |
GCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT > NC_000913/2189656‑2189917
|
gCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAg < 2:223847/139‑1 (MQ=255)
cGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAg < 2:731099/124‑1 (MQ=255)
cGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAg > 1:731099/1‑124 (MQ=255)
ccGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa > 2:42919/1‑139 (MQ=255)
aTGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATaa < 2:692860/114‑1 (MQ=255)
aTGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATaa > 1:692860/1‑114 (MQ=255)
aTGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAAccc > 2:243117/1‑139 (MQ=255)
cGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGcc < 1:42919/139‑1 (MQ=255)
aGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACa > 2:348645/1‑139 (MQ=255)
tCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACa < 2:22765/132‑1 (MQ=255)
tCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACa > 1:22765/1‑132 (MQ=255)
aCGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACttt < 1:243117/139‑1 (MQ=255)
aTGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGc > 1:155722/1‑139 (MQ=255)
aaTGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCt > 1:659644/1‑139 (MQ=255)
gtgtATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAat < 2:706291/139‑1 (MQ=255)
ttCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGc > 1:255074/1‑139 (MQ=255)
cagccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGAcc < 2:155722/139‑1 (MQ=255)
agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa < 2:632039/71‑1 (MQ=255)
agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa < 2:178253/71‑1 (MQ=255)
agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa > 1:632039/1‑71 (MQ=255)
agccCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTaa > 1:178253/1‑71 (MQ=255)
cccccAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGGCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCCGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTAAGTCccgc > 2:590511/1‑139 (MQ=255)
cccccAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACccgc > 2:581681/1‑139 (MQ=255)
taataaTTATTCGCGACCATCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCg > 2:481661/1‑139 (MQ=255)
tCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGc > 2:25975/1‑79 (MQ=255)
tCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGc < 1:25975/79‑1 (MQ=255)
tCGAGCCACCATGCAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCat < 1:590511/139‑1 (MQ=255)
|
GCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTGTATTCCAGCCAGCCCCCAAAGTAAACGCGTAATAATTATTCGCGACCATCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCACTTTGTTTGCTCGCCCCTTCAATATGGCTACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT > NC_000913/2189656‑2189917
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
