Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
77 |
35.1 |
1569012 |
96.6% |
1515665 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,748,181 |
G→A |
A366A (GCG→GCA) |
lyxK → |
L‑xylulose kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,748,181 | 0 | G | A | 100.0%
| 105.9
/ NA
| 31 | A366A (GCG→GCA) | lyxK | L‑xylulose kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/22); total (9/22) |
TCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAA > NC_000913/3748051‑3748316
|
tcaatcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGtt > 1:134909/1‑139 (MQ=255)
atcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCa < 2:369404/139‑1 (MQ=255)
caGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg < 1:491275/139‑1 (MQ=255)
aGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGc < 1:585424/139‑1 (MQ=255)
ggCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGcc < 1:745130/139‑1 (MQ=255)
aGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAg < 2:584797/139‑1 (MQ=255)
ttGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTTTGAAgggg < 1:509993/139‑1 (MQ=255)
ttGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAgggg < 2:392369/139‑1 (MQ=255)
gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggtg < 1:330918/139‑1 (MQ=255)
gAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGgtggtg < 1:190553/139‑1 (MQ=255)
aGGGTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGtt < 1:426984/139‑1 (MQ=255)
cTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg < 1:602978/139‑1 (MQ=255)
gggCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCat < 1:197549/139‑1 (MQ=255)
aTCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc > 2:649495/1‑139 (MQ=255)
tctcTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGTCATCTATGAAGGGGTGGTGTTCAGCCATATGAcc < 2:269209/139‑1 (MQ=255)
tctcTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAcc < 2:177072/139‑1 (MQ=255)
ccTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg < 1:143141/86‑1 (MQ=255)
ccTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg > 2:143141/1‑86 (MQ=255)
tGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGa < 1:649495/139‑1 (MQ=255)
gTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGaa < 1:140697/139‑1 (MQ=255)
cGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggt < 1:368014/96‑1 (MQ=255)
cGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggt > 2:368014/1‑96 (MQ=255)
aCGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg < 1:755639/72‑1 (MQ=255)
aCGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGa < 2:69012/139‑1 (MQ=255)
aCGCCGGACTCGAGATGACCAGGGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg > 2:755639/1‑72 (MQ=255)
cTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTcaca > 2:734309/1‑139 (MQ=255)
gACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGa < 2:570542/66‑1 (MQ=255)
gACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGa > 1:570542/1‑66 (MQ=255)
aTGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCAt > 2:369612/1‑41 (MQ=255)
aTGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCAt < 1:369612/41‑1 (MQ=255)
aTGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGgcgc > 2:123765/1‑139 (MQ=255)
cgcacACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCaa > 2:556529/1‑139 (MQ=255)
|
TCAATCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAA > NC_000913/3748051‑3748316
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
