Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	67	36.0	1614110	95.1%	1535018	114.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,748,181	G→A	A366A (GCG→GCA)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,181	0	G	A	100.0%	111.7 / NA	33	A366A (GCG→GCA)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/16); total (17/16)

TCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGC  >  NC_000913/3748055‑3748314

tcaGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAg                                                                                                                           <  2:294792/139‑1 (MQ=255)

     ccGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAATGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCAt                                                                                                                      >  1:5967/1‑139 (MQ=255)

                 tGCCGAAGGCTGGGGGCGATCTCTTTTCCCTGCCGGTCCTGTACGTCAGCCACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATATATGAAGGggt                                                                                                          <  2:342854/139‑1 (MQ=255)

                  gCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggtg                                                                                                         >  1:393302/1‑139 (MQ=255)

                          cTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAg                                                                                                 <  2:309645/139‑1 (MQ=255)

                             ggggCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCa                                                                                              <  2:393302/139‑1 (MQ=255)

                                          tttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc                                                                                        <  2:705829/132‑1 (MQ=255)

                                          tttCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGAc                                                                                        >  1:705829/1‑132 (MQ=255)

                                             ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg                                                                                                                          <  1:307480/95‑1 (MQ=255)

                                             ccTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAgg                                                                                                                          >  2:307480/1‑95 (MQ=255)

                                                gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTc                                                                                                   <  1:32503/115‑1 (MQ=255)

                                                gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTc                                                                                                   >  2:32503/1‑115 (MQ=255)

                                                gCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                                                                           >  1:40515/1‑139 (MQ=255)

                                                    ttCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggtggt                                                                                                       >  1:696090/1‑107 (MQ=255)

                                                    ttCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGggtggt                                                                                                       <  2:696090/107‑1 (MQ=255)

                                                      ccTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATgcgc                                                                     <  1:58758/139‑1 (MQ=255)

                                                         gTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGaa                                                                  <  2:477911/139‑1 (MQ=255)

                                                               cagcaACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGtttt                                                            >  2:37527/1‑139 (MQ=255)

                                                                agcaACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                                                                           >  1:312564/1‑123 (MQ=255)

                                                                agcaACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGa                                                                           <  2:312564/123‑1 (MQ=255)

                                                                          gACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTca                                                 <  2:201340/139‑1 (MQ=255)

                                                                                     aCCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                                                                                   <  2:284603/94‑1 (MQ=255)

                                                                                     aCCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCAcc                                                                                   >  1:284603/1‑94 (MQ=255)

                                                                                     aCCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTAcgcg                                      >  2:85372/1‑139 (MQ=255)

                                                                                               tCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGcc                                                                                                                        >  2:102237/1‑47 (MQ=255)

                                                                                               tCTACGGGATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGcc                                                                                                                        <  1:102237/47‑1 (MQ=255)

                                                                                                      gATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAATGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGgcg                     <  2:570846/139‑1 (MQ=255)

                                                                                                      gATGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGgcg                     >  2:780596/1‑139 (MQ=255)

                                                                                                        tGCAGGCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCa                   >  2:319709/1‑139 (MQ=255)

                                                                                                             gCCATTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCg              <  1:319709/139‑1 (MQ=255)

                                                                                                                aTTCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGTCCGGCGCACTCCGATg           >  1:153328/1‑139 (MQ=255)

                                                                                                                  tCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTc         >  2:461593/1‑139 (MQ=255)

                                                                                                                  tCACACCCGCGCACACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTc         >  2:662420/1‑139 (MQ=255)

                                                                                                                         cgcgcaCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGc  <  1:461593/139‑1 (MQ=255)

TCAGGCCGTTGCCAGCTTGCCGAAGGCTGGGGGCGATCTCTTTTTCCTGCCGTTCCTGTACGGCAGCAACGCCGGACTCGAGATGACCAGTGGTTTCTACGGGATGCAGGCCATTCACACCCGCGCGCACCTGTTGCAGGCCATCTATGAAGGGGTGGTGTTCAGCCATATGACCCACCTCAACCGAATGCGCGAACGTTTTACTGATGTTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGC  >  NC_000913/3748055‑3748314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A