Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
77 |
35.1 |
1569012 |
96.6% |
1515665 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,136,860 |
A→G |
T251T (ACA→ACG) |
yijE → |
EamA‑like transporter family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,136,860 | 0 | A | G | 100.0%
| 91.3
/ NA
| 26 | T251T (ACA→ACG) | yijE | EamA‑like transporter family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/13); total (13/13) |
GTGTTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC > NC_000913/4136753‑4136995
|
gtgtTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCt > 2:631751/1‑110 (MQ=255)
gtgtTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCt < 1:631751/110‑1 (MQ=255)
gcgcTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGgc > 1:317210/1‑139 (MQ=255)
cGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATcc > 2:79960/1‑139 (MQ=255)
cGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATcc > 2:729319/1‑139 (MQ=255)
cGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATcc > 1:733602/1‑139 (MQ=255)
ggCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGaa > 1:201636/1‑139 (MQ=255)
tGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAg < 2:317210/139‑1 (MQ=255)
gCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCg < 2:607084/139‑1 (MQ=255)
tGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTTGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTa < 1:680769/139‑1 (MQ=255)
tATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGtt > 2:678850/1‑62 (MQ=255)
tATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGtt < 1:678850/62‑1 (MQ=255)
ggTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTattgtgctgat < 2:201636/139‑1 (MQ=255)
tttgtATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg < 1:763715/106‑1 (MQ=255)
tttgtATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg > 2:763715/1‑106 (MQ=255)
ttGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCAc < 1:729319/139‑1 (MQ=255)
aaaCTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg < 1:256601/95‑1 (MQ=255)
aaaCTTGCCTGCCAGTATTGCCAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCggggg > 2:256601/1‑95 (MQ=255)
ccAGCTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa > 1:522709/1‑139 (MQ=255)
cTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAg > 2:402124/1‑84 (MQ=255)
cTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAg < 1:402124/84‑1 (MQ=255)
cTTAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa < 2:201891/139‑1 (MQ=255)
ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg < 1:715343/112‑1 (MQ=255)
ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg > 2:715343/1‑112 (MQ=255)
ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga > 2:588374/1‑137 (MQ=255)
ttAAGCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga < 1:588374/137‑1 (MQ=255)
gCACGCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGACGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt < 2:522709/139‑1 (MQ=255)
cacgCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc < 2:733602/139‑1 (MQ=255)
|
GTGTTCTGGGCGCTGGCCTACAGTGCGATTCTGGCGACGGCACTGGCGTGGAGCTTATGGTTGTTTGTATTGAAAAACTTGCCTGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC > NC_000913/4136753‑4136995
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
