Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
77 |
35.1 |
1569012 |
96.6% |
1515665 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,186,296 |
T→A |
I316N (ATC→AAC) |
rpoC → |
RNA polymerase, beta prime subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,186,296 | 0 | T | A | 100.0%
| 111.9
/ NA
| 33 | I316N (ATC→AAC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (17/16); total (17/16) |
CGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT > NC_000913/4186160‑4186423
|
cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACa > 1:14852/1‑139 (MQ=255)
gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac < 1:336506/139‑1 (MQ=255)
gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac < 2:485493/139‑1 (MQ=255)
cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac < 2:328345/138‑1 (MQ=255)
cgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAacac > 1:328345/1‑138 (MQ=255)
aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGc > 1:441446/1‑139 (MQ=255)
tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc > 1:401930/1‑139 (MQ=255)
tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc > 1:624852/1‑139 (MQ=255)
ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc < 2:401930/139‑1 (MQ=255)
ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGAc < 2:14852/139‑1 (MQ=255)
tCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATg > 1:350440/1‑139 (MQ=255)
cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa > 2:575294/1‑139 (MQ=255)
tacgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGttt > 2:463723/1‑139 (MQ=255)
gtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCg > 1:72659/1‑139 (MQ=255)
cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg > 1:328300/1‑139 (MQ=255)
aCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGCTCTAACAAGCGTCCTCTGAAATCTTTGGCCGTCATGATCAAAGGTAAACAGGGTCGTGTCCGTCAGAACCTg < 1:476830/139‑1 (MQ=255)
gctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGt < 2:755806/61‑1 (MQ=255)
gctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGt > 1:755806/1‑61 (MQ=255)
ctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa < 2:441446/139‑1 (MQ=255)
gAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgtg < 2:624852/139‑1 (MQ=255)
aGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGACCTCTGAAATCTTTGGCCGACATGATCAAAGATAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGtt > 2:509487/1‑139 (MQ=255)
aTAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt > 1:650358/1‑70 (MQ=255)
aTAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt < 2:650358/70‑1 (MQ=255)
tAACGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa < 1:575294/139‑1 (MQ=255)
aCGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAAt < 2:328300/139‑1 (MQ=255)
cGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTc > 2:355286/1‑103 (MQ=255)
cGGTCGTCGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTc < 1:355286/103‑1 (MQ=255)
gtcgtcGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGactac < 2:11660/119‑1 (MQ=255)
gtcgtcGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGactac > 1:11660/1‑119 (MQ=255)
gtcgtcGCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCAc > 2:269175/1‑139 (MQ=255)
gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt < 1:763213/107‑1 (MQ=255)
gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt > 2:763213/1‑107 (MQ=255)
gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt < 1:5752/139‑1 (MQ=255)
gtcgCGGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGt < 2:72659/139‑1 (MQ=255)
cgGTCGTGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGTTCGTTCTGTAATCACCGTAGGt < 2:350440/139‑1 (MQ=255)
tGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt > 2:376640/1‑51 (MQ=255)
tGCGAACACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGAt < 1:376640/51‑1 (MQ=255)
|
CGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATCACCGTAGGT > NC_000913/4186160‑4186423
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
