Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I1 R1 77 35.1 1569012 96.6% 1515665 112.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,533,223 A→G intergenic (‑40/+16) yjhP ← / ← yjhQ putative methyltransferase/GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,533,2230AG100.0% 192.1 / NA 50intergenic (‑40/+16)yjhP/yjhQputative methyltransferase/GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/21);  total (29/21)

GCGACCCAGCGTGGCGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTTGTGCTCCTCCGGGATGGGATA  >  NC_000913/4533094‑4533358
                                                                                                                                 |                                                                                                                                       
gcgACCCAGCGTGGCGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGAt                                                                                                                                <  2:196415/139‑1 (MQ=255)
  gACCCAGCGTGGCGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTg                                                                                                                              <  1:262061/139‑1 (MQ=255)
              cGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGcaacaa                                                                                                                                   >  1:294650/1‑122 (MQ=255)
              cGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGcaacaa                                                                                                                                   <  2:294650/122‑1 (MQ=255)
              cGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCa                                                                                                                  >  1:752011/1‑139 (MQ=255)
              cGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATGGGGTTAAGCCTAAAAAAGAACGCAACAAGATTGTTATTCACGTCa                                                                                                                  >  2:274630/1‑139 (MQ=255)
                       ctTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCgg                                                                                                         >  1:482576/1‑139 (MQ=255)
                          cGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGttt                                                                                                      >  2:21058/1‑139 (MQ=255)
                             tGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGa                                                                                                                                 >  1:696641/1‑109 (MQ=255)
                             tGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGa                                                                                                                                 <  2:696641/109‑1 (MQ=255)
                             tGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTtcat                                                                                                   >  2:203726/1‑139 (MQ=255)
                               aaCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACgcaac                                                                                                                                     <  2:179899/103‑1 (MQ=255)
                               aaCGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACgcaac                                                                                                                                     >  1:179899/1‑103 (MQ=255)
                                             gCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCg                                                                                   >  1:234412/1‑139 (MQ=255)
                                              cGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCt                                                                                                             >  1:704708/1‑112 (MQ=255)
                                              cGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCt                                                                                                             <  2:704708/112‑1 (MQ=255)
                                              cGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCa                                                                                      >  1:315264/1‑135 (MQ=255)
                                              cGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCa                                                                                      <  2:315264/135‑1 (MQ=255)
                                              cGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCgg                                                                                  >  1:557853/1‑139 (MQ=255)
                                                   ttCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGAtgtg                                                                             >  2:120509/1‑139 (MQ=255)
                                                    tCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTgg                                                                            >  2:695851/1‑139 (MQ=255)
                                                                aTGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTc                                                                >  2:529234/1‑139 (MQ=255)
                                                                    tAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATgg                                                            >  1:398413/1‑139 (MQ=255)
                                                                      aaaaTACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGc                                                          <  1:203726/139‑1 (MQ=255)
                                                                          tACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAg                                                      <  2:482576/139‑1 (MQ=255)
                                                                            cGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCg                                                    >  1:161419/1‑139 (MQ=255)
                                                                            cGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCg                                                    >  1:396907/1‑139 (MQ=255)
                                                                             gTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCgg                                                   >  2:520675/1‑139 (MQ=255)
                                                                              tGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGgt                                                  >  1:382943/1‑139 (MQ=255)
                                                                                  atatCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGgtgagt                                              <  2:557853/139‑1 (MQ=255)
                                                                                     tCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAg                                                                                                                 <  2:434358/69‑1 (MQ=255)
                                                                                     tCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAg                                                                                                                 >  1:434358/1‑69 (MQ=255)
                                                                                        attattTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGc                                        >  1:551318/1‑139 (MQ=255)
                                                                                               aaTTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCa                                                                                      >  2:598494/1‑86 (MQ=255)
                                                                                               aaTTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCa                                                                                      <  1:598494/86‑1 (MQ=255)
                                                                                               aaTTCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATcc                                 >  2:666407/1‑139 (MQ=255)
                                                                                                 ttCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCcag                               >  1:484454/1‑139 (MQ=255)
                                                                                                  tCCTGTTATTGGGTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCcagc                              <  2:752011/139‑1 (MQ=255)
                                                                                                   tcctttttaaggTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAg                                                      >  1:378377/11‑114 (MQ=255)
                                                                                                   tcctttttaaggTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAg                                                      <  2:378377/104‑1 (MQ=255)
                                                                                                            gggTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGgtg                                                 <  2:94371/110‑1 (MQ=255)
                                                                                                            gggTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGgtg                                                 >  1:94371/1‑110 (MQ=255)
                                                                                                            gggTTAAGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTtgtg                    <  2:396907/139‑1 (MQ=255)
                                                                                                                 aaGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTgg                                                                            >  2:321309/1‑78 (MQ=255)
                                                                                                                 aaGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTgg                                                                            <  1:321309/78‑1 (MQ=255)
                                                                                                                 aaGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCAt                                                              >  1:506514/1‑92 (MQ=255)
                                                                                                                 aaGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTATGTGctcct               >  1:501032/1‑139 (MQ=255)
                                                                                                                 aaGCCTTAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAAGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCAt                                                              <  2:506514/92‑1 (MQ=255)
                                                                                                                       tAAAAAGGACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTTGTGCTCCTCCggga         <  1:695851/139‑1 (MQ=255)
                                                                                                                       tAAAAAGGACGCAACAAGATTGTTATTCAAGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATAGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTTGTGCTCCTCCggga         <  1:529234/139‑1 (MQ=255)
                                                                                                                              gACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTTGTGc                   <  2:376089/122‑1 (MQ=255)
                                                                                                                              gACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTTGTGc                   >  1:376089/1‑122 (MQ=255)
                                                                                                                              gACGCAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTTGTGCTCCTCCGGGATGGGATa  <  2:234412/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                       
GCGACCCAGCGTGGCGTACTTCTCTTCGGTGAACGGGTTATGGATGCGGTGTTCACTTTCGCTGATGGTAAAAATACGTGGGATATCCATTATTTAATTCCTGTTATTGGGTTAAGCCTTAAAAAGGACACAACAAGATTGTTATTCACGTCAGTGCTCCGGTTTCATCAGGGTCTGCGCACCGGATGTGGCCGGTCAGGGTCATGGGCTGAGCGGTGAGTGATTGCATCATCCAGCAGGCTTTGTGCTCCTCCGGGATGGGATA  >  NC_000913/4533094‑4533358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A