Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
69 |
53.0 |
2252012 |
96.1% |
2164183 |
115.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,533,530 |
C→T |
L85L (CTG→CTA) |
yjhQ ← |
GNAT family putative N‑acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,533,530 | 0 | C | T | 95.0%
| 121.1
/ ‑2.0
| 40 | L85L (CTG→CTA) | yjhQ | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (16/22); total (17/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
CGAACCCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCT > NC_000913/4533394‑4533663
|
cGAACGCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCAGCCCTTGGCATTCCAGTATGACCGCTAg < 2:409422/139‑1 (MQ=255)
aaCCCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAggg < 2:904579/139‑1 (MQ=255)
cATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGc < 2:26804/139‑1 (MQ=255)
aTGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCg < 2:979189/139‑1 (MQ=255)
tAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCa < 1:913546/139‑1 (MQ=255)
tggcgtggcCGAGAAC‑AAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGt < 1:100248/139‑1 (MQ=255)
tggcCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAAt > 1:899266/1‑139 (MQ=255)
agaACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCAttt < 2:194056/139‑1 (MQ=255)
gCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGcc < 1:513272/139‑1 (MQ=255)
gCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCCTATCAACCCTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGcc < 2:137880/139‑1 (MQ=255)
gCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg < 2:634058/139‑1 (MQ=255)
gCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg < 1:890423/139‑1 (MQ=255)
aTCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCAGGTATGACCGCTAGGGGAGCGAgg > 2:566555/1‑90 (MQ=255)
aTCAACCTCAGGTGCTCGAGGCCAGTGCGTATCAACCGGCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAgg < 1:566555/90‑1 (MQ=255)
tCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGa > 1:128821/1‑139 (MQ=255)
tCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGa > 2:38087/1‑139 (MQ=255)
tATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCa > 1:291294/1‑139 (MQ=255)
cAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc < 2:127704/139‑1 (MQ=255)
cAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc < 1:8084/139‑1 (MQ=255)
ccGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCgg > 1:773588/1‑139 (MQ=255)
gTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGc > 1:648009/1‑139 (MQ=255)
tcctccCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGaa > 2:1045762/1‑70 (MQ=255)
tcctccCACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGaa < 1:1045762/70‑1 (MQ=255)
ccACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg < 2:959151/92‑1 (MQ=255)
ccACGCCCATCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCgg > 1:959151/1‑92 (MQ=255)
aTCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc > 1:1094882/1‑120 (MQ=255)
aTCCCTTGGTATTCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATAc < 2:1094882/120‑1 (MQ=255)
tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTg < 1:3425/67‑1 (MQ=255)
tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTg > 2:3425/1‑67 (MQ=255)
tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCtt > 2:360647/1‑97 (MQ=255)
tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCtt < 1:360647/97‑1 (MQ=255)
tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcgcgc > 1:525625/1‑139 (MQ=255)
tCCGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGgcgcgcgc > 1:1115297/1‑139 (MQ=255)
cGGTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCtt > 1:484656/1‑139 (MQ=255)
ggTATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCttt < 1:884512/139‑1 (MQ=255)
tATGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCa < 2:773588/139‑1 (MQ=255)
tATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCa < 1:38087/139‑1 (MQ=255)
tGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc < 2:1115297/139‑1 (MQ=255)
tGACCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATc > 2:111282/1‑139 (MQ=255)
aCCGCTAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCt > 1:909725/1‑139 (MQ=255)
|
CGAACCCATGTCGGGGGTAATAGGTGGCGTGGCCGAGAACAAAAACCGTCTGGCAGCCCATCAACCTCAGGTGCTCGATGCCAGTGCGTATCAACCGTCCTCCCACGCCCATCCCTTGGTATTCCGGTATGACCGCCAGGGGAGCGAGGATGTGCATCAACGGTGAATCCATTTCTCCTTTGAAGGTTGCCCGGGTAAATAAAATATGCCCGACGGCTTTGCCTTCATACCGGGCTAGCAGCGATAACGCGGGGCGCGCGCTTTCATCCT > NC_000913/4533394‑4533663
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
