Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I2 R1 59 52.6 2411032 94.1% 2268781 113.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 640,165 C→T A138V (GCG→GTG)  ahpF → alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913640,1650CT100.0% 170.8 / NA 49A138V (GCG→GTG) ahpFalkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/26);  total (23/26)

GCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCAGTGTTCGTAAACGGGAAAGAG  >  NC_000913/640034‑640292
                                                                                                                                   |                                                                                                                               
gCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGTCGTGGTGCAGGTGCTGAAc                                                                                                                          >  1:1106139/1‑139 (MQ=255)
gCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAAc                                                                                                                          >  1:1059934/1‑139 (MQ=255)
           gtggtCATCCGTCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAAc                                                                                                                          <  1:924066/128‑1 (MQ=255)
           gtggtCATCCGTCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAAc                                                                                                                          >  2:924066/1‑128 (MQ=255)
                 aTCCGTCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGaa                                                                                                         >  2:869308/1‑139 (MQ=255)
                      tCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGTCGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCcgc                                                                                                    <  2:1106139/139‑1 (MQ=255)
                      tCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCcgc                                                                                                    <  1:37150/139‑1 (MQ=255)
                             aagCGCAGTCTCTGCTGGAGCAGATTCGCTATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCaa                                                                                             >  2:480829/1‑139 (MQ=255)
                                       tctGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGca                                                                                          <  2:518518/132‑1 (MQ=255)
                                       tctGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGca                                                                                          >  1:518518/1‑132 (MQ=255)
                                           ctgGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTg                                                                               <  2:771867/139‑1 (MQ=255)
                                               aGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCtgat                                                                                                                     >  1:982177/1‑97 (MQ=255)
                                               aGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCtgat                                                                                                                     <  2:982177/97‑1 (MQ=255)
                                                   gATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACggcggc                                                                       >  1:219901/1‑139 (MQ=255)
                                                    aTTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCa                                                                      <  1:549887/139‑1 (MQ=255)
                                                                       aTTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCAc                                                   >  1:435954/1‑139 (MQ=255)
                                                                       aTTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCAc                                                   >  2:1088329/1‑139 (MQ=255)
                                                                           tGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCgcgc                                                                                                  <  2:233854/88‑1 (MQ=255)
                                                                           tGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCgcgc                                                                                                  >  1:233854/1‑88 (MQ=255)
                                                                           tGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGAt                                               <  1:1088329/139‑1 (MQ=255)
                                                                              aTTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGc                                            <  2:1059934/139‑1 (MQ=255)
                                                                               ttCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCtg                                                                                                                       >  2:494193/1‑63 (MQ=255)
                                                                               ttCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCtg                                                                                                                       <  1:494193/63‑1 (MQ=255)
                                                                                          ttACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGc                                >  1:271973/1‑139 (MQ=255)
                                                                                          ttACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCT‑AACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCg                               <  1:591148/139‑1 (MQ=255)
                                                                                                gCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCg                          >  2:665606/1‑139 (MQ=255)
                                                                                                gCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCg                          <  1:665606/139‑1 (MQ=255)
                                                                                                 ctctctTGCCACAACTGCCCGGACGTGGTGCAGGTGCTg                                                                                                                             <  1:731275/39‑1 (MQ=255)
                                                                                                 ctctctTGCCACAACTGCCCGGACGTGGTGCAGGTGCTg                                                                                                                             >  2:731275/1‑39 (MQ=255)
                                                                                                 ctctctTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCCCCGATCGCAACGTGATCGGCGTTCCgg                         <  2:582576/139‑1 (MQ=255)
                                                                                                   ctctTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCa                       <  1:842675/139‑1 (MQ=255)
                                                                                                    tctTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCa                       >  1:1088481/1‑138 (MQ=255)
                                                                                                    tctTGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCa                       <  2:1088481/138‑1 (MQ=255)
                                                                                                       tGCCACAACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCCGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCAgtgt                   >  1:161731/1‑139 (MQ=255)
                                                                                                            caACTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCAGTGTTCGTa              <  1:480829/139‑1 (MQ=255)
                                                                                                              aCTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca                                                                                        <  2:740570/63‑1 (MQ=255)
                                                                                                              aCTGCCCGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGcaca                                                                                        >  1:740570/1‑63 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCgcgc                                                                                                  >  2:143673/1‑49 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCgcgc                                                                                                  <  1:143673/49‑1 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGca                                                                                          >  2:472757/1‑57 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGca                                                                                          <  1:472757/57‑1 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCa                                                                      <  2:1029053/77‑1 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCa                                                                      >  1:1029053/1‑77 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCa                                           <  1:972488/104‑1 (MQ=255)
                                                                                                                  cccGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCa                                           >  2:972488/1‑104 (MQ=255)
                                                                                                                    cGGACGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCAGTGTTCGTAAACGGGaa      <  1:919300/139‑1 (MQ=255)
                                                                                                                        cGTGGTGCAGGTGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCAGTGTTCGTAAACGGGAAagag  <  2:271973/139‑1 (MQ=255)
                                                                                                                             tgCAGGTGCTGAACCTGATGAGCGTACTGAACCcgc                                                                                                    <  2:1111865/36‑1 (MQ=255)
                                                                                                                             tgCAGGTGCTGAACCTGATGAGCGTACTGAACCcgc                                                                                                    >  1:1111865/1‑36 (MQ=255)
                                                                                                                                   |                                                                                                                               
GCTGTGGACCGGTGGTCATCCGTCGAAAGAAGCGCAGTCTCTGCTGGAGCAGATTCGCCATATTGACGGTGATTTTGAATTCGAAACCTATTACTCGCTCTCTTGCCACAACTGCCCGGACGTGGTGCAGGCGCTGAACCTGATGAGCGTACTGAACCCGCGCATCAAGCACACTGCAATTGACGGCGGCACCTTCCAGAACGAAATCACCGATCGCAACGTGATGGGCGTTCCGGCAGTGTTCGTAAACGGGAAAGAG  >  NC_000913/640034‑640292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A