Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
20.9 |
912876 |
97.7% |
891879 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
640,862 |
G→A |
A370A (GCG→GCA) |
ahpF → |
alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 640,862 | 0 | G | A | 100.0%
| 44.0
/ NA
| 14 | A370A (GCG→GCA) | ahpF | alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/7); total (7/7) |
CAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATT > NC_000913/640733‑640979
|
cAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGAc > 2:224123/1‑139 (MQ=255)
gCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACgctgct > 2:283920/1‑139 (MQ=255)
cACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTaa > 1:63889/1‑106 (MQ=255)
cACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTaa < 2:63889/106‑1 (MQ=255)
gCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCt < 1:224123/139‑1 (MQ=255)
cTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGTGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAgg < 1:283920/139‑1 (MQ=255)
gTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAAtt > 1:326634/1‑53 (MQ=255)
gTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAAtt < 2:326634/53‑1 (MQ=255)
aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGa > 1:61374/1‑70 (MQ=255)
aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGa < 2:61374/70‑1 (MQ=255)
aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAg > 1:207102/1‑139 (MQ=255)
aGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAgg > 1:58185/1‑119 (MQ=255)
aGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAgg < 2:58185/119‑1 (MQ=255)
cggTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACattatt < 2:207102/139‑1 (MQ=255)
|
CAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATT > NC_000913/640733‑640979
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
