Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	65	21.2	1002576	96.5%	967485	111.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	640,862	G→A	A370A (GCG→GCA)	ahpF →	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	640,862	0	G	A	100.0%	44.8 / NA	14	A370A (GCG→GCA)	ahpF	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/9); total (5/9)

TGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAA  >  NC_000913/640728‑640964

tGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCaa                                                                                                    <  2:200133/139‑1 (MQ=255)

     cAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGAc                                                                                               <  1:182191/139‑1 (MQ=255)

             aCGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCggg                                                                                       <  2:448316/139‑1 (MQ=255)

               gTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTa                                                                                     <  1:185225/139‑1 (MQ=255)

                                        ccAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGa                                                            >  2:3065/1‑139 (MQ=255)

                                                        cTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATg                                            >  2:308077/1‑139 (MQ=255)

                                                                aCTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGtt                                                                                <  1:406852/95‑1 (MQ=255)

                                                                aCTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGtt                                                                                >  2:406852/1‑95 (MQ=255)

                                                                aCTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCACCAGAAATGAAAGCCGa                                    <  2:313710/139‑1 (MQ=255)

                                                                       cGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGtt                             <  2:157140/139‑1 (MQ=255)

                                                                                        gTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTgcg            <  1:159241/139‑1 (MQ=255)

                                                                                                  aGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGaaa  >  2:76008/1‑139 (MQ=255)

                                                                                                                                  aaGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGc                                       <  1:113991/70‑1 (MQ=255)

                                                                                                                                  aaGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGc                                       >  2:113991/1‑70 (MQ=255)

TGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAA  >  NC_000913/640728‑640964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A