Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	59	52.6	2411032	94.1%	2268781	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,907	G→A	Q353* (CAA→TAA)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,907	0	G	A	100.0%	151.9 / NA	44	Q353* (CAA→TAA)	dacA	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/24); total (20/24)

ACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTGCAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGATCAGAATCAC  >  NC_000913/662772‑663029

aCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCAGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAg                                                                                                                         >  2:1198455/1‑139 (MQ=255)

   ttaatttaatGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAgcgg                                                                                                                      >  2:832455/1‑139 (MQ=255)

    taatttaatGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAgcggc                                                                                                                     >  1:1180871/1‑139 (MQ=255)

      atttaatGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGgcgc                                                                                                                   >  1:943972/1‑139 (MQ=255)

            tGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCaa                                                                                                             >  2:912429/1‑139 (MQ=255)

                aTCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                <  2:537223/132‑1 (MQ=255)

                aTCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                >  1:537223/1‑132 (MQ=255)

                   aaTGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCActg                                                                                                      <  2:921753/139‑1 (MQ=255)

                        ttttGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGtt                                                                                                 <  2:943972/139‑1 (MQ=255)

                           tGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCCCTGCTGTTCAg                                                                                              >  1:786110/1‑139 (MQ=255)

                                                           aaCACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGAc                                                              >  1:512594/1‑139 (MQ=255)

                                                            acacAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGAcc                                                             >  2:494022/1‑139 (MQ=255)

                                                             cacaACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCa                                                            <  2:967939/139‑1 (MQ=255)

                                                                    aGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGaa                                                     <  2:649549/139‑1 (MQ=255)

                                                                     gCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAAt                                                    >  1:890638/1‑139 (MQ=255)

                                                                      cGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATg                                                   >  1:1039643/1‑139 (MQ=255)

                                                                       gggCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATgg                                                  >  1:439463/1‑139 (MQ=255)

                                                                        ggCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGt                                                 <  2:890638/139‑1 (MQ=255)

                                                                        ggCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGt                                                 >  2:1171754/1‑139 (MQ=255)

                                                                         gCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTc                                                <  1:602571/139‑1 (MQ=255)

                                                                          cGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                >  1:897435/1‑74 (MQ=255)

                                                                          cGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                <  2:897435/74‑1 (MQ=255)

                                                                           gTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAg                                              <  2:17426/139‑1 (MQ=255)

                                                                                tCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTaca                                         <  2:439463/139‑1 (MQ=255)

                                                                                   aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCGGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      <  1:85274/139‑1 (MQ=255)

                                                                                   aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      <  2:786110/139‑1 (MQ=255)

                                                                                   aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      >  2:977494/1‑139 (MQ=255)

                                                                                   aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      >  2:107635/1‑139 (MQ=255)

                                                                                   aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      <  1:494022/139‑1 (MQ=255)

                                                                                     cGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCt                                    <  2:1013816/139‑1 (MQ=255)

                                                                                       ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCa                                                                                                         >  1:414786/1‑68 (MQ=255)

                                                                                       ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCa                                                                                                         <  2:414786/68‑1 (MQ=255)

                                                                                       ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTc                                                                                                <  1:1126831/77‑1 (MQ=255)

                                                                                       ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTc                                                                                                >  2:1126831/1‑77 (MQ=255)

                                                                                         ttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTAt                                <  2:1180871/139‑1 (MQ=255)

                                                                                           gCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCa                              <  2:1039643/139‑1 (MQ=255)

                                                                                                         aGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCa                              <  2:862327/125‑1 (MQ=255)

                                                                                                         aGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCa                              >  1:862327/1‑125 (MQ=255)

                                                                                                         aGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAgc                <  1:1171754/139‑1 (MQ=255)

                                                                                                          gTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGCCAGGTACACGTCTTTATCAACCCCTAACGAAgcg               <  1:912429/139‑1 (MQ=255)

                                                                                                            tGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGcgcg             <  1:832455/139‑1 (MQ=255)

                                                                                                              aTAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGAt           >  1:363801/1‑139 (MQ=255)

                                                                                                                       acgacCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGaa                                                     <  2:53270/88‑1 (MQ=255)

                                                                                                                       acgacCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGaa                                                     >  1:53270/1‑88 (MQ=255)

                                                                                                                       acgacCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGATCAGAATCac  <  1:977494/139‑1 (MQ=255)

ACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTGCAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGATCAGAATCAC  >  NC_000913/662772‑663029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A