Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	59	52.6	2411032	94.1%	2268781	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,464,156	T→C	intergenic (+86/+96)	yfdF → / ← mlaA	uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,464,156	0	T	C	100.0%	152.9 / NA	42	intergenic (+86/+96)	yfdF/mlaA	uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/18); total (24/18)

AATGAAATTAATCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCT  >  NC_000913/2464020‑2464283

aaTGAAATTAATCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCt                                                                                                                               <  1:510039/139‑1 (MQ=255)

          aTCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGt                                                                                                                     >  1:1081318/1‑139 (MQ=255)

          aTCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGt                                                                                                                     <  1:490576/139‑1 (MQ=255)

                    tGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAtct                                                                                                           >  2:1045144/1‑139 (MQ=255)

                           ggTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTa                                                                                                    >  2:34693/1‑139 (MQ=255)

                             tgtTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTAGGATTTTTCAACAGCTGAATATCGGTAATAGGCAGTGACGAATGTCAATATTTGTACATAATTACGCCTAGGTCATTGGCGGTTGATCTCCTTTTACt                                                                                                  <  1:1195538/139‑1 (MQ=255)

                              gtTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACtt                                                                                                 >  2:476921/1‑139 (MQ=255)

                                   ttttGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGt                                                                                            <  1:476921/139‑1 (MQ=255)

                                       gatgatATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             >  1:614090/1‑118 (MQ=255)

                                       gatgatATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             <  2:614090/118‑1 (MQ=255)

                                             atTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAtct                                                                                                           >  1:460665/1‑114 (MQ=255)

                                             atTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAtct                                                                                                           <  2:460665/114‑1 (MQ=255)

                                             atTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGa                                                                                  >  2:952249/1‑139 (MQ=255)

                                              tttAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGAt                                                                                 <  1:34693/139‑1 (MQ=255)

                                                       attTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACaaa                                                                        >  1:951002/1‑139 (MQ=255)

                                                       attTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACaaa                                                                        >  1:999674/1‑139 (MQ=255)

                                                                  tttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACt                                                                                                  >  1:430101/1‑102 (MQ=255)

                                                                  tttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACt                                                                                                  <  2:430101/102‑1 (MQ=255)

                                                                           tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             >  1:1199221/1‑82 (MQ=255)

                                                                           tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             <  2:1199221/82‑1 (MQ=255)

                                                                                    cTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttttt                                           >  1:877466/1‑139 (MQ=255)

                                                                                    cTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttttt                                           >  2:1053082/1‑139 (MQ=255)

                                                                                        atatCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAttt                                       <  2:877466/139‑1 (MQ=255)

                                                                                                    gTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGTTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCa                           >  2:197750/1‑139 (MQ=255)

                                                                                                      cAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGa                         <  2:951002/139‑1 (MQ=255)

                                                                                                         tGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATc                      >  1:29813/1‑139 (MQ=255)

                                                                                                         tGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATc                      >  2:934503/1‑139 (MQ=255)

                                                                                                             gAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAata                  <  1:1053082/139‑1 (MQ=255)

                                                                                                              aaCATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTa                                                                                                              <  1:752875/46‑1 (MQ=255)

                                                                                                              aaCATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTa                                                                                                              >  2:752875/1‑46 (MQ=255)

                                                                                                              aaCATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAatat                 >  1:39257/1‑139 (MQ=255)

                                                                                                                   cAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                             >  1:82453/1‑106 (MQ=255)

                                                                                                                   cAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                             <  2:82453/106‑1 (MQ=255)

                                                                                                                   cAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtttt            >  2:232417/1‑139 (MQ=255)

                                                                                                                      tATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaaa         <  2:39257/139‑1 (MQ=255)

                                                                                                                             aCATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACt                                                     >  2:461852/1‑88 (MQ=255)

                                                                                                                             aCATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACt                                                     <  1:461852/88‑1 (MQ=255)

                                                                                                                             aCATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCt  >  2:864361/1‑139 (MQ=255)

                                                                                                                              cATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGTGACt                                                     >  2:1022884/1‑87 (MQ=255)

                                                                                                                              cATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGTGACt                                                     <  1:1022884/87‑1 (MQ=255)

                                                                                                                                tAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCa                                                   <  2:650422/87‑1 (MQ=255)

                                                                                                                                tAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCa                                                   >  1:650422/1‑87 (MQ=255)

AATGAAATTAATCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCT  >  NC_000913/2464020‑2464283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A