Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	59	52.6	2411032	94.1%	2268781	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,189	G→A	intergenic (‑58/+32)	bacA ← / ← folB	undecaprenyl pyrophosphate phosphatase/dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,189	0	G	A	100.0%	158.0 / NA	47	intergenic (‑58/+32)	bacA/folB	undecaprenyl pyrophosphate phosphatase/dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/17); total (30/17)

ATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGG  >  NC_000913/3204056‑3204322

aTACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTtat                                                                                                                                  >  2:525399/1‑139 (MQ=255)

      gCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGt                                                                                                                            >  2:1049691/1‑139 (MQ=255)

      gCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATAACCAAGTTATAACCGt                                                                                                                            >  1:830176/1‑139 (MQ=255)

      gCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGAGTATGACCAAGTTATAACCGt                                                                                                                            >  1:711519/1‑139 (MQ=255)

       cAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGtt                                                                                                                           <  2:133511/139‑1 (MQ=255)

             ttCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGttt                                                                                                                     <  2:128503/139‑1 (MQ=255)

                       ccTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTa                                                                                                           <  2:179531/139‑1 (MQ=255)

                                  acCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAattat                                                                                                >  2:639772/1‑139 (MQ=255)

                                            gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCa                                                                                       <  2:392820/139‑1 (MQ=255)

                                            gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCa                                                                                       >  1:392820/1‑139 (MQ=255)

                                            gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  >  2:81559/1‑111 (MQ=255)

                                            gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  <  1:81559/111‑1 (MQ=255)

                                            gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAg                                                                                      >  2:754428/1‑139 (MQ=255)

                                             ccGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGa                                                                                     >  1:1029927/1‑139 (MQ=255)

                                                cTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGatta                                                                                  >  2:727618/1‑139 (MQ=255)

                                                cTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGatta                                                                                  >  1:1033029/1‑139 (MQ=255)

                                                      gcagcGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCa                                                                            <  1:238007/139‑1 (MQ=255)

                                                           gAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCa                                                                            >  2:1127783/1‑134 (MQ=255)

                                                           gAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCa                                                                            <  1:1127783/134‑1 (MQ=255)

                                                           gAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTc                                                                       <  1:727618/139‑1 (MQ=255)

                                                                 atatCGCTCATCAATAAACCCCTAACTTATT‑AACATGTACCGCTTGGCCGAACTACTGCGTATGACCAAGTTATAAGCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCATGCTCAATGAt                                                                 >  2:1080883/1‑139 (MQ=255)

                                                                 atatCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATAACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGAt                                                                 <  2:830176/139‑1 (MQ=255)

                                                                       cTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  <  2:629083/84‑1 (MQ=255)

                                                                       cTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  >  1:629083/1‑84 (MQ=255)

                                                                         catcaATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGcc                                                                             <  2:1020029/119‑1 (MQ=255)

                                                                         catcaATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGcc                                                                             >  1:1020029/1‑119 (MQ=255)

                                                                          atcaATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAAc                                                        >  1:357134/1‑139 (MQ=255)

                                                                               tAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTcg                                                   >  2:135751/1‑139 (MQ=255)

                                                                                    cccTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGat                                                                                    <  2:151493/101‑1 (MQ=255)

                                                                                    cccTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGat                                                                                    >  1:151493/1‑101 (MQ=255)

                                                                                       tAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAg                                                                                                                <  1:356907/70‑1 (MQ=255)

                                                                                       tAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAg                                                                                                                >  2:356907/1‑70 (MQ=255)

                                                                                        aaaTTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCcgcg                                          <  2:1033029/139‑1 (MQ=255)

                                                                                         aaTTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCgcgc                                         >  1:159915/1‑139 (MQ=255)

                                                                                         aaTTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCgcgc                                         >  2:743853/1‑139 (MQ=255)

                                                                                                    aTGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCt                               <  2:159915/139‑1 (MQ=255)

                                                                                                       tACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGc                            >  2:962353/1‑139 (MQ=255)

                                                                                                         ccGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCtt                          >  2:621202/1‑139 (MQ=255)

                                                                                                         ccGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCtt                          >  2:1007358/1‑139 (MQ=255)

                                                                                                         ccGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCACCACTGCGCCTGGCtt                          >  1:854453/1‑139 (MQ=255)

                                                                                                         ccGCTTGTCCGAACTACTGAGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCtt                          >  2:449520/1‑139 (MQ=255)

                                                                                                                 ccGAACTACTGCGTCTGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGAGTATTGCCACGCTCAATGATTACGCCAGCATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGtt                  >  1:733904/1‑139 (MQ=255)

                                                                                                                    aaCTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGa               >  1:1091264/1‑139 (MQ=255)

                                                                                                                        actGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACg           <  1:754428/139‑1 (MQ=255)

                                                                                                                               aTGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCAc    >  1:741241/1‑139 (MQ=255)

                                                                                                                                tGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCACCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACg   <  2:854453/139‑1 (MQ=255)

                                                                                                                                 gACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACgg  >  2:353393/1‑139 (MQ=255)

ATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGG  >  NC_000913/3204056‑3204322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A