Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
58 |
36.1 |
1832140 |
90.7% |
1661750 |
105.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,437,231 |
(T)9→8 |
intergenic (+2/+29) |
micC → / ← pfo |
sRNA antisense regulator of OmpC translation, Hfq‑dependent/pyruvate‑flavodoxin oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,437,223 | 0 | T | . | 100.0%
| 156.1
/ NA
| 37 | noncoding (103/109 nt) | micC | sRNA antisense regulator of OmpC translation, Hfq‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (18/19); total (18/19) |
CTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTG > NC_000913/1437101‑1437357
|
cTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGa > 2:237608/1‑139 (MQ=255)
tataCGTTATTCTGCGCGGGTTATATGCCTCTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt < 1:290208/139‑1 (MQ=255)
cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATcc > 1:297606/1‑130 (MQ=255)
cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATcc < 2:297606/130‑1 (MQ=255)
cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCaga > 1:806554/1‑139 (MQ=255)
cgcgGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCaga > 2:226537/1‑139 (MQ=255)
gTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGATCAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATACTTATCCAGAGCAtt > 2:578398/1‑139 (MQ=255)
ttATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCAt < 2:901360/127‑1 (MQ=255)
ttATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCAt > 1:901360/1‑127 (MQ=255)
ttATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGtt > 1:670571/1‑139 (MQ=255)
tATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCagag < 2:536096/123‑1 (MQ=255)
tATTGTCACAGATTTTATTTGCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCagag > 1:536096/1‑123 (MQ=255)
tCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATc < 2:739102/107‑1 (MQ=255)
tCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATc > 1:739102/1‑107 (MQ=255)
tattttCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCtttt < 1:393348/139‑1 (MQ=255)
gTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCAt < 2:806554/139‑1 (MQ=255)
tGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCAttt < 1:237608/139‑1 (MQ=255)
tgcCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt < 2:498049/70‑1 (MQ=255)
tgcCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt > 1:498049/1‑70 (MQ=255)
ccACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCgg > 2:338539/1‑118 (MQ=255)
ccACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCgg < 1:338539/118‑1 (MQ=255)
ccACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCAt > 1:497861/1‑139 (MQ=255)
tCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCa < 1:33713/70‑1 (MQ=255)
tCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCa > 2:33713/1‑70 (MQ=255)
ccAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGc < 1:198685/139‑1 (MQ=255)
aCAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATcc < 2:289912/47‑1 (MQ=255)
aCAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATcc > 1:289912/1‑47 (MQ=255)
cAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCAt < 1:797560/139‑1 (MQ=255)
cAAGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCAt < 1:724366/139‑1 (MQ=255)
aGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt < 1:408658/39‑1 (MQ=38)
aGCCCGAACAGTCGTCCGGGC‑TTTTTTTTAGAATTGGAt > 2:408658/1‑39 (MQ=38)
aCAGTCGTCCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATa < 2:670571/139‑1 (MQ=255)
tcgtcCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCAGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTg > 1:289753/1‑139 (MQ=255)
cgtcCGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTGTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGt < 1:578398/139‑1 (MQ=255)
cGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTgcag < 2:332747/118‑1 (MQ=255)
cGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTgcag > 1:332747/1‑118 (MQ=255)
cGGGC‑TTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTg > 2:895998/1‑139 (MQ=255)
|
CTATACGTTATTCTGCGCGGGTTATATGCCTTTATTGTCACAGATTTTATTTTCTGTTGGGCCATTGCATTGCCACTGATTTTCCAACATATAAAAAGACAAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTG > NC_000913/1437101‑1437357
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
