Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	63	64.8	2934156	93.7%	2749304	111.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,443,966	C→T	Q306* (CAA→TAA)	ydbH →	putative membrane‑anchored protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,443,966	0	C	T	100.0%	127.4 / NA	37	Q306* (CAA→TAA)	ydbH	putative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/22); total (15/22)

AAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGC  >  NC_000913/1443833‑1444096

aaGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCg                                                                                                                               <  2:838788/139‑1 (MQ=255)

             tGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAAcg                                                                                                                  <  2:920200/139‑1 (MQ=255)

             tGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAAcg                                                                                                                  <  2:634272/139‑1 (MQ=255)

             tGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAAcg                                                                                                                  <  2:1163901/139‑1 (MQ=255)

                    ggCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGGGGGTAAGGGCAACGCGGTGCt                                                                                                           <  2:1071042/139‑1 (MQ=255)

                         tATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAAtt                                                                                                      >  2:255392/1‑139 (MQ=255)

                            gCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAggg                                                                                                                       <  1:187531/119‑1 (MQ=255)

                            gCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAggg                                                                                                                       >  2:187531/1‑119 (MQ=255)

                                   ttCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAgg                                                                                            <  1:255392/139‑1 (MQ=255)

                                          gAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTg                                                                                                             <  2:590969/115‑1 (MQ=255)

                                          gAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTg                                                                                                             >  1:590969/1‑115 (MQ=255)

                                             tGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGc                                                                                  <  2:1067902/139‑1 (MQ=255)

                                               gCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCAt                                                                                >  2:396214/1‑139 (MQ=255)

                                                ccGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATg                                                                               >  2:1132688/1‑139 (MQ=255)

                                                      gggTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAAc                                                                         <  1:1345970/139‑1 (MQ=255)

                                                                        cTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAg                                                       <  1:598252/139‑1 (MQ=255)

                                                                               gcaggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTATGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCg                                                <  2:950041/139‑1 (MQ=255)

                                                                                cagtGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACGCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCgg                                               <  1:1239847/139‑1 (MQ=255)

                                                                                                tctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCg                               <  1:1114715/139‑1 (MQ=255)

                                                                                                 ctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCgg                              <  1:279847/139‑1 (MQ=255)

                                                                                                  tGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                      <  1:656230/130‑1 (MQ=255)

                                                                                                  tGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                      >  2:656230/1‑130 (MQ=255)

                                                                                                         cGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAg                                  <  1:508540/127‑1 (MQ=255)

                                                                                                         cGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAg                                  >  2:508540/1‑127 (MQ=255)

                                                                                                              gacTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            <  1:930734/128‑1 (MQ=255)

                                                                                                              gacTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGAATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            >  2:930734/1‑128 (MQ=255)

                                                                                                                   aGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTT‑ATTTTGGTCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTatat                <  2:867447/134‑1 (MQ=255)

                                                                                                                   aGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTT‑ATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTatat                >  1:867447/1‑134 (MQ=255)

                                                                                                                          tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAAtt                                                                                                      <  2:212437/42‑1 (MQ=255)

                                                                                                                          tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAAtt                                                                                                      >  1:212437/1‑42 (MQ=255)

                                                                                                                          tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            <  1:1058269/116‑1 (MQ=255)

                                                                                                                          tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            >  2:1058269/1‑116 (MQ=255)

                                                                                                                          tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCA‑GGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTGCCt    >  1:945774/1‑139 (MQ=255)

                                                                                                                             cccAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgc  >  2:839284/1‑139 (MQ=255)

                                                                                                                             cccAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgc  >  2:409655/1‑139 (MQ=255)

                                                                                                                                 ggggTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGc                                                      >  1:942391/1‑83 (MQ=255)

                                                                                                                                 ggggTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGc                                                      <  2:942391/83‑1 (MQ=255)

AAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGC  >  NC_000913/1443833‑1444096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A