Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	58	36.1	1832140	90.7%	1661750	105.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,481,041	A→G	T45A (ACG→GCG)	ynbD →	putative phosphatase inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,481,041	0	A	G	100.0%	136.7 / NA	36	T45A (ACG→GCG)	ynbD	putative phosphatase inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/18); total (18/18)

TATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTA  >  NC_000913/1480907‑1481175

tATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGc                                                                                                                                    <  1:441575/139‑1 (MQ=255)

 aTTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAgcggcg                                                                                                                                   <  1:136031/139‑1 (MQ=255)

        cAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCtt                                                                                                                            >  2:430296/1‑139 (MQ=255)

          aGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCtttt                                                                                                                          >  1:894757/1‑139 (MQ=255)

               ctggctggTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTcc                                                                                                                     <  1:191213/139‑1 (MQ=255)

                  gctggTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTg                                                                                                                  <  1:430296/139‑1 (MQ=255)

                  gctggTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTg                                                                                                                  >  1:153927/1‑139 (MQ=255)

                          ttgttgCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTg                                                                                                          <  2:894757/139‑1 (MQ=255)

                            gttgCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        <  2:153927/139‑1 (MQ=255)

                                cTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCtt                                                                                                    >  1:406272/1‑139 (MQ=255)

                                         tttttCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGa                                                                                                                                  >  1:427320/1‑100 (MQ=255)

                                         tttttCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGa                                                                                                                                  <  2:427320/100‑1 (MQ=255)

                                           tttCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTg                                                                                         <  2:406272/139‑1 (MQ=255)

                                                                          ttCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGttt                                                          >  2:329661/1‑139 (MQ=255)

                                                                          ttCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTAGCTGTTCtttt                                                          >  2:592885/1‑139 (MQ=255)

                                                                            cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTgg                                                                                        >  1:406555/1‑107 (MQ=255)

                                                                            cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTgg                                                                                        <  2:406555/107‑1 (MQ=255)

                                                                                 cgGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCAc                                                   <  1:592885/139‑1 (MQ=255)

                                                                                    ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        >  2:260457/1‑83 (MQ=255)

                                                                                    ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        <  1:260457/83‑1 (MQ=255)

                                                                                    ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACt                                                                                                 >  1:743038/1‑90 (MQ=255)

                                                                                    ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACt                                                                                                 <  2:743038/90‑1 (MQ=255)

                                                                                              tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        >  1:875211/1‑73 (MQ=255)

                                                                                              tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        <  2:875211/73‑1 (MQ=255)

                                                                                              tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAg                                      >  2:436636/1‑139 (MQ=255)

                                                                                                         atatCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGt                           >  2:901087/1‑139 (MQ=255)

                                                                                                            tCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAgcg                                    >  1:720183/1‑127 (MQ=255)

                                                                                                            tCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAgcg                                    <  2:720183/127‑1 (MQ=255)

                                                                                                                 aGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATc                                                                                     <  2:135196/73‑1 (MQ=255)

                                                                                                                 aGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATc                                                                                     >  1:135196/1‑73 (MQ=255)

                                                                                                                 aGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGTTTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGc                   >  1:492333/1‑139 (MQ=255)

                                                                                                                   tCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTcc                                                                                                      <  2:50672/54‑1 (MQ=255)

                                                                                                                   tCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTcc                                                                                                      >  1:50672/1‑54 (MQ=255)

                                                                                                                      ggTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAtt              <  1:901087/139‑1 (MQ=255)

                                                                                                                           tCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                              >  2:496026/1‑118 (MQ=255)

                                                                                                                           tCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                              <  1:496026/118‑1 (MQ=255)

                                                                                                                                  ggAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTa  <  1:436636/139‑1 (MQ=255)

TATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTA  >  NC_000913/1480907‑1481175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A