Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
58 |
36.1 |
1832140 |
90.7% |
1661750 |
105.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,027,398 |
C→T |
W207* (TGG→TAG) |
yedQ ← |
putative membrane‑anchored diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,027,398 | 0 | C | T | 100.0%
| 119.5
/ NA
| 35 | W207* (TGG→TAG) | yedQ | putative membrane‑anchored diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/18); total (17/18) |
CATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAG > NC_000913/2027264‑2027517
|
cATACCAGTAGTTATTACTGTATACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAggg < 1:231403/139‑1 (MQ=255)
gtTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACa > 2:866989/1‑139 (MQ=255)
ttattaCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAATCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACAt < 2:363746/139‑1 (MQ=255)
attaCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAg < 1:866989/139‑1 (MQ=255)
taCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGcc > 2:80535/1‑139 (MQ=255)
tCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACAt < 1:159023/130‑1 (MQ=255)
tCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACAt > 2:159023/1‑130 (MQ=255)
tCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGtaat > 2:449353/1‑139 (MQ=255)
ccGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAAcg > 2:308497/1‑139 (MQ=255)
ccGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAAcg > 2:10951/1‑139 (MQ=255)
ggTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTg < 2:353799/139‑1 (MQ=255)
aCCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGcgcg > 2:659420/1‑139 (MQ=255)
cTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAggg > 2:3734/1‑80 (MQ=255)
cTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAggg < 1:3734/80‑1 (MQ=255)
cTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCg > 1:581741/1‑139 (MQ=255)
gtgtTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGaa > 1:546026/1‑139 (MQ=255)
ttCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGAc > 1:300933/1‑81 (MQ=255)
ttCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGAc < 2:300933/81‑1 (MQ=255)
cGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCg < 1:263451/139‑1 (MQ=255)
gAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAAcgcg < 1:633432/88‑1 (MQ=255)
gAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAAcgcg > 2:633432/1‑88 (MQ=255)
gTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCAc < 1:274750/139‑1 (MQ=255)
gAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCAcgc < 1:659420/139‑1 (MQ=255)
cAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCgaga < 2:546026/139‑1 (MQ=255)
cAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCgaga < 1:10951/139‑1 (MQ=255)
cgcgGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAAcgcg < 2:877960/69‑1 (MQ=255)
cgcgGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAAcgcg > 1:877960/1‑69 (MQ=255)
cgGTGACGATTTTCTCGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCt > 2:843711/1‑139 (MQ=255)
ctcGTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATcga > 1:144103/1‑139 (MQ=255)
gTTGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATcgacga < 2:581741/139‑1 (MQ=255)
ttGTGAATGGCCAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACgag < 1:80535/139‑1 (MQ=255)
ccAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATc > 2:3047/1‑108 (MQ=255)
ccAATAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATc < 1:3047/108‑1 (MQ=255)
aTAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACAtt < 2:200392/47‑1 (MQ=255)
aTAAACTAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACAtt > 1:200392/1‑47 (MQ=255)
|
CATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAG > NC_000913/2027264‑2027517
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
