Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
58 |
36.1 |
1832140 |
90.7% |
1661750 |
105.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,697,352 |
A→G |
N518D (AAT→GAT) |
mltF → |
membrane‑bound lytic transglycosylase F, murein hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,697,352 | 0 | A | G | 100.0%
| 149.1
/ NA
| 39 | N518D (AAT→GAT) | mltF | membrane‑bound lytic transglycosylase F, murein hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/18); total (21/18) |
ATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAATTTCCACTCGGTGATTCATACCCG > NC_000913/2697218‑2697487
|
aTCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTa < 1:859075/139‑1 (MQ=255)
aTCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTa > 2:859411/1‑139 (MQ=255)
cggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGt > 2:599918/1‑139 (MQ=255)
aGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCttt > 2:426698/1‑139 (MQ=255)
ttGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCttttt > 1:823062/1‑139 (MQ=255)
tGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCtttttt < 2:8811/139‑1 (MQ=255)
ggCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCt > 2:503999/1‑139 (MQ=255)
ttCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTggcg > 1:107695/1‑139 (MQ=255)
gtcgtcATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCa > 1:138172/1‑139 (MQ=255)
atcaAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGc > 1:594925/1‑139 (MQ=255)
cccATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCttt > 2:792194/1‑88 (MQ=255)
cccATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCttt < 1:792194/88‑1 (MQ=255)
ccATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCa < 2:594925/139‑1 (MQ=255)
cATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCAt < 2:138172/139‑1 (MQ=255)
tctcCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTgcg > 2:869403/1‑79 (MQ=255)
tctcCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTgcg < 1:869403/79‑1 (MQ=255)
tctctGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGAtt < 1:486748/58‑1 (MQ=255)
tctctGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGAtt > 2:486748/1‑58 (MQ=255)
ctgctgTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTAttc > 1:212784/1‑139 (MQ=255)
gTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg > 2:856018/1‑84 (MQ=255)
gTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:856018/84‑1 (MQ=255)
gTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTAttccttcc > 2:777665/1‑139 (MQ=255)
gTTTTCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTAttccttcc < 2:908290/139‑1 (MQ=255)
ttttCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCa < 2:579460/85‑1 (MQ=255)
ttttCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCa > 1:579460/1‑85 (MQ=255)
tttCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCg < 2:264214/88‑1 (MQ=255)
tttCCAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCg > 1:264214/1‑88 (MQ=255)
cAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAAc > 2:135349/1‑103 (MQ=255)
cAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAAc < 1:135349/103‑1 (MQ=255)
cAGGAAAGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAAtt > 1:402866/1‑139 (MQ=255)
aaaGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAATTTCCa < 2:402866/139‑1 (MQ=255)
aaGGGAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAATTTCCAc < 1:503999/139‑1 (MQ=255)
gggAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCg > 2:776675/1‑77 (MQ=255)
gggAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCg < 1:776675/77‑1 (MQ=255)
ggAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAATTTCCACTca < 1:426698/139‑2 (MQ=255)
gAGTGAAGAGAAACAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAATTTCCACTCgg < 2:823062/139‑1 (MQ=255)
aaCAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATc > 1:819982/1‑97 (MQ=255)
aaCAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATc < 2:819982/97‑1 (MQ=255)
cAAGATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAATTTCCACTCGGTGATTCATACCCg > 2:452500/1‑139 (MQ=255)
|
ATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCAACGCCGCGCACTCATCCGCCAGTATTCCTTCCGTAATTTCCACTCGGTGATTCATACCCG > NC_000913/2697218‑2697487
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
