Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	78	48.0	2243616	94.8%	2126947	114.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,704,009	G→A	H19Y (CAT→TAT)	rnc ←	RNase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,704,009	0	G	A	100.0%	104.1 / NA	31	H19Y (CAT→TAT)	rnc	RNase III
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/14); total (17/14)

GAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCG  >  NC_000913/2703880‑2704141

gAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAgt                                                                                                                             >  2:653861/1‑139 (MQ=255)

 aGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGTAACAGTTCCTGATAATTAAAAgtg                                                                                                                            >  1:853952/1‑139 (MQ=255)

  gggAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAgtgt                                                                                                                           >  1:428687/1‑139 (MQ=255)

   ggAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTa                                                                                                                          >  2:344129/1‑139 (MQ=255)

            gATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGGTGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCtt                                                                                                                 <  2:208213/139‑1 (MQ=255)

                 aGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGtt                                                                                                            >  1:902509/1‑139 (MQ=255)

                     cATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAg                                                                                                        <  2:902509/139‑1 (MQ=255)

                              aaaCGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGattaat                                                                                               <  2:503832/138‑1 (MQ=255)

                                            aTAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCa                                                                                 >  2:1084156/1‑139 (MQ=255)

                                              agagTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGTAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATg                                                                               <  2:853952/139‑1 (MQ=255)

                                                     ccTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGCTGGCGTTCATGCGATAcc                                                                        >  2:686558/1‑139 (MQ=255)

                                                     ccTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                        <  1:80818/139‑1 (MQ=255)

                                                     ccTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                        >  1:694385/1‑139 (MQ=255)

                                                     ccTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                        >  2:707845/1‑139 (MQ=255)

                                                     ccTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                        >  2:740669/1‑139 (MQ=255)

                                                     ccTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATAcc                                                                        >  2:52615/1‑139 (MQ=255)

                                                                                  aCTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGcac                                           <  1:1084156/139‑1 (MQ=255)

                                                                                     gctgGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACg                                        >  2:359065/1‑139 (MQ=255)

                                                                                       tgGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGCTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGaa                                      >  1:309473/1‑139 (MQ=255)

                                                                                        ggCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGa                                                                                          <  2:62161/86‑1 (MQ=255)

                                                                                        ggCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGa                                                                                          >  1:62161/1‑86 (MQ=255)

                                                                                        ggCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGaaa                                     <  1:52615/139‑1 (MQ=255)

                                                                                        ggCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGaaa                                     <  1:344129/139‑1 (MQ=255)

                                                                                         gCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAAc                                    >  2:920971/1‑139 (MQ=255)

                                                                                             tACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGAc                                <  2:501836/139‑1 (MQ=255)

                                                                                             tACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGAc                                <  2:428821/139‑1 (MQ=255)

                                                                                              aCGATGAGTTAATGCCTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGAcc                               <  2:476917/139‑1 (MQ=255)

                                                                                                            ccTGCTGCAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGtt                 <  1:920971/139‑1 (MQ=255)

                                                                                                                ctgcAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAg                                  >  2:490873/1‑118 (MQ=255)

                                                                                                                ctgcAACAGTTCCTGATAATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAg                                  <  1:490873/118‑1 (MQ=255)

                                                                                                                           ccTGATAACTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCg  >  2:1005685/1‑139 (MQ=255)

                                                                                                                              gataatTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTg                             <  2:951176/109‑1 (MQ=255)

GAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGCACTACGATGAGTTAATGCCTGCTGCAACAGTTCCTGATGATTAAAAGTGTAGCCCAGCTTCCGTTGAAGCCGATTAATTACGATGGGGTTCATGCGATACCAATAAATAAATGCGTCAACAATTCAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCG  >  NC_000913/2703880‑2704141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A