Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 67 31.4 1402038 96.1% 1347358 109.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,706,066 T→C E353E (GAA→GAG)  lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,706,0660TC100.0% 61.1 / NA 17E353E (GAA→GAG) lepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

GATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAA  >  NC_000913/2705937‑2706197
                                                                                                                                 |                                                                                                                                   
gATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGgatg                                                                                                                            <  1:512971/139‑1 (MQ=255)
                              gCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCg                                                                                              <  1:556828/139‑1 (MQ=255)
                                                     ttGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcgc                                                                       >  1:172521/1‑139 (MQ=255)
                                                                     ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCGGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                                                       <  1:59566/139‑1 (MQ=255)
                                                                              tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAc                                              >  2:77342/1‑139 (MQ=255)
                                                                              tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAc                                              >  1:195142/1‑139 (MQ=255)
                                                                                   tCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGa                                         >  2:373314/1‑139 (MQ=255)
                                                                                      gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc                                      <  1:373314/139‑1 (MQ=255)
                                                                                                 aTCAGATCCAGATCGTATTCACGTTCCAGACGCTcc                                                                                                                                  <  1:231757/36‑1 (MQ=255)
                                                                                                 aTCAGATCCAGATCGTATTCACGTTCCAGACGCTcc                                                                                                                                  >  2:231757/1‑36 (MQ=255)
                                                                                                 aTCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                           <  1:77342/139‑1 (MQ=255)
                                                                                                        ccagatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc                                      >  1:187486/1‑121 (MQ=255)
                                                                                                        ccagatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc                                      <  2:187486/121‑1 (MQ=255)
                                                                                                           gatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                                                                       >  1:155806/1‑69 (MQ=255)
                                                                                                           gatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                                                                       <  2:155806/69‑1 (MQ=255)
                                                                                                                tATTCAGGTTCCAGATTCTCCCGGATGATCGCCATTGGTAGCAGGAAGGGGAAGCCGCATCGGAAACCCAAGCCCAGCGCGCTGAAGCTTTCCGGCTCCTAGAACAGTGAGGCATCGTTCAGGCTCAGTTTACCCAgcg            >  1:394460/1‑139 (MQ=255)
                                                                                                                        ttCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACgg    >  1:103733/1‑139 (MQ=255)
                                                                                                                          ccAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa  <  2:195142/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
GATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAA  >  NC_000913/2705937‑2706197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A