Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	69	53.0	2252012	96.1%	2164183	115.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,720,122	T→C	V57A (GTG→GCG)	pka →	protein lysine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,720,122	0	T	C	100.0%	134.5 / NA	36	V57A (GTG→GCG)	pka	protein lysine acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/20); total (16/20)

AATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAACCTGCATTAT  >  NC_000913/2719990‑2720248

aaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggt                                                                                                                          <  1:1028437/139‑2 (MQ=255)

aaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggc                                                                                                                          <  2:610077/139‑1 (MQ=255)

aaTCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggc                                                                                                                          <  1:1005379/139‑1 (MQ=255)

              aTTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTg                                                                                                            <  2:244623/139‑1 (MQ=255)

                  gcgcGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggcc                                                                                                                         >  2:705241/1‑122 (MQ=255)

                  gcgcGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTtggcc                                                                                                                         <  1:705241/122‑1 (MQ=255)

                  gcgcGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAg                                                                                                        >  1:124184/1‑139 (MQ=255)

                   cgcgTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGAGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGc                                                                                                       <  2:707261/139‑1 (MQ=255)

                      gTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCCGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCCGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTg                                                                                                    >  2:824963/1‑139 (MQ=255)

                       tCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGc                                                                                                   >  2:530087/1‑139 (MQ=255)

                                         gcAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTg                                                                                 <  2:295013/139‑1 (MQ=255)

                                           aGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                                                               <  1:912616/139‑1 (MQ=255)

                                           aGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCg                                                                               <  1:272305/139‑1 (MQ=255)

                                              ttACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGtt                                                                            <  1:288262/139‑1 (MQ=255)

                                                 ccTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTa                                                                         <  1:911049/139‑1 (MQ=255)

                                                       gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTAcc                                                                   >  2:259074/1‑139 (MQ=255)

                                                       gatgCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTAcc                                                                   >  1:511725/1‑139 (MQ=255)

                                                           cGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATg                                                               >  2:851454/1‑139 (MQ=255)

                                                            gTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGc                                                              <  2:124184/139‑1 (MQ=255)

                                                                   gctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCt                                                                                   <  2:974618/111‑1 (MQ=255)

                                                                   gctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCt                                                                                   >  1:974618/1‑111 (MQ=255)

                                                                    ctgGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTa                                                      >  2:412289/1‑139 (MQ=255)

                                                                       gCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATc                                                   >  1:492780/1‑139 (MQ=255)

                                                                          ggAGGCTTTGACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTg                                                <  2:662152/139‑1 (MQ=255)

                                                                                        aCCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAagag                                  <  1:851454/139‑1 (MQ=255)

                                                                                                 cccGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCgag                         <  1:259074/139‑1 (MQ=255)

                                                                                                  ccGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCgaga                        >  1:204546/1‑139 (MQ=255)

                                                                                                    ggTGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGaaa                      >  1:55367/1‑139 (MQ=255)

                                                                                                      tGACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAgg                    <  1:894791/139‑1 (MQ=255)

                                                                                                       gACGCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGc                   >  1:521409/1‑139 (MQ=255)

                                                                                                          gCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCtt                                                                                                     >  2:1065868/1‑54 (MQ=255)

                                                                                                          gCCAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCtt                                                                                                     <  1:1065868/54‑1 (MQ=255)

                                                                                                            cAGCCTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTaa              >  1:416186/1‑139 (MQ=255)

                                                                                                               ccTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAAc           <  2:511725/139‑1 (MQ=255)

                                                                                                               ccTGGAAAGCGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAAc           <  1:824963/139‑1 (MQ=255)

                                                                                                                        cGGTGTTGGGTGCGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAACCTGCattat  >  2:1120983/1‑139 (MQ=255)

AATCGATAGCGGTAATTGGCGCGTCGATGAAACCCAATCGCGCAGGTTACCTGATGATGCGTAACCTGCTGGCGGGAGGCTTTAACGGACCGGTACTCCCGGTGACGCCAGCCTGGAAAGCGGTGTTGGGTGTGTTGGCCTGGCCGGATATTGCCAGCTTGCCCTTTACACCCGACCTTGCGGTTTTATGTACCAATGCCAGCCGTAATCTTGCTCTTCTGGAAGAGCTCGGCGAGAAAGGCTGTAAAACCTGCATTAT  >  NC_000913/2719990‑2720248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A